Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,293,171	G→A	intergenic (‑249/‑356)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,293,171	0	G	A	100.0%	56.7 / NA	16	intergenic (‑249/‑356)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/9);  total (7/9)

CCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATG  >  NC_000913/1293100‑1293232                                                                        |                                                              ccGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGAcc                                                      >  2:232646/1‑81 (MQ=255)   gatgatTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCtt                                                    >  2:232647/1‑81 (MQ=255)            gcagcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGtcttc                                           >  1:232653/1‑81 (MQ=255)             cagcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGtcttct                                          <  1:232626/81‑1 (MQ=255)               gcaCGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGt                                        <  1:232627/81‑1 (MQ=255)                      aGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGt                                  <  1:232628/80‑1 (MQ=255)                         ttAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTtgtg                              <  1:232629/81‑1 (MQ=255)                         ttAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTtgtg                              >  2:232649/1‑81 (MQ=255)                            aaaCTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGttt                           <  2:232630/81‑1 (MQ=255)                              aCTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTAc                         >  2:232650/1‑81 (MQ=255)                                      aTTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAAt                   <  1:232631/79‑1 (MQ=255)                                      aTTCATGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTc                 <  1:232632/81‑1 (MQ=255)                                          aTGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTa             <  2:232633/81‑1 (MQ=255)                                          aTGTCACATTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTa             >  2:232655/1‑81 (MQ=255)                                                 aTTTTATGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGc       >  2:232651/1‑80 (MQ=255)                                                      aTGGGGAGATTATCGTAGACTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAatg  <  1:232634/80‑1 (MQ=255)                                                                        |                                                              CCGATGATTTCGCAGCACGTGAGGTTAAAACTTCCTGATTCATGTCACATTTTATGGGGAGATTATCGTAGGCTGACGACCTTTCAGTCTTCTGTATTAGTTGTGTTTACGAGAATTCCCTATTAAGCGAATG  >  NC_000913/1293100‑1293232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑