Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,299,534	T→C	F2L (TTT→CTT)	insH21 →	IS5 transposase and trans‑activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,299,534	0	T	C	100.0%	26.1 / NA	9	F2L (TTT→CTT)	insH21	IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/2);  total (7/2)

GATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT  >  NC_000913/1299457‑1299565                                                                              |                                gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTg                              >  1:233521/1‑81 (MQ=255)              ccACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGTTATGAGATCATGCTTGTCATCTGGAGCCa                 >  2:233524/1‑81 (MQ=255)               cACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCAt                <  1:233497/81‑1 (MQ=255)               cACTGCCTTTGGTAGAGGAAAGTGCT‑AGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTTATTTGGAGCCa                 >  1:233522/1‑79 (MQ=18)                aCTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCa                 >  1:233518/1‑79 (MQ=255)                       ttGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGTGCCATAGAACAgg        >  2:233520/1‑81 (MQ=255)                           tAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGtt     >  2:233516/1‑80 (MQ=255)                             gagGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcat  <  1:233498/81‑1 (MQ=255)                             gagGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGCTTGTCATCTGGAGCCATAGAACAGGGTtcat  >  2:233525/1‑81 (MQ=255)                                                                              |                                GATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCAT  >  NC_000913/1299457‑1299565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑