Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,307,693	G→A	P318L (CCG→CTG)	clsA ←	cardiolipin synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,307,693	0	G	A	100.0%	54.1 / NA	15	P318L (CCG→CTG)	clsA	cardiolipin synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/4);  total (11/4)

GGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAA  >  NC_000913/1307620‑1307756                                                                          |                                                                gggCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGc                                                           <  2:234707/80‑1 (MQ=255)   gCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCgg                                                        >  1:234737/1‑81 (MQ=255)        gTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGcc                                                    <  2:234708/80‑1 (MQ=255)        gTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGcc                                                    <  2:234709/80‑1 (MQ=255)        gTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGc                                                     >  2:234735/1‑79 (MQ=255)                     atTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATtgtgt                                      >  1:234724/1‑81 (MQ=255)                            cgcCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGgt                               <  2:234710/81‑1 (MQ=255)                                gAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGa                           >  1:234727/1‑81 (MQ=255)                                gAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGa                           >  1:234736/1‑81 (MQ=255)                                 aaTAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGAc                          >  1:234732/1‑81 (MQ=255)                                    aaGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGc                       >  1:234728/1‑81 (MQ=255)                                    aaGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGc                       >  2:234742/1‑81 (MQ=255)                                         gCAGTCAATAATGCCTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGcc                  >  1:234733/1‑81 (MQ=255)                                                      ccTGGTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCa     >  1:234726/1‑81 (MQ=255)                                                         ggTGAATGAGATCTTCCAGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCataa  >  1:234734/1‑81 (MQ=255)                                                                          |                                                                GGGCGTGGTCATGATCAAATATTCACGCGCCGAATAAGCCGCAGTCAATAATGCCTGGTGAATGAGATCTTCCGGAAAGCCGGGGCCAGAAGCAATTGTGTGAATGGTGTGACCGCTGGCCTGTTCAAACGGCATAA  >  NC_000913/1307620‑1307756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑