| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,349,230 | T→G | K293T (AAA→ACA) | yciW ← | putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,349,230 | 0 | T | G | 78.6% | 30.9 / 2.7 | 14 | K293T (AAA→ACA) | yciW | putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base T (2/1); new base G (8/3); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
CGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATAACAAC > NC_000913/1349158‑1349301 | cgcTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCa < 2:240548/81‑1 (MQ=255) cTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCAtt < 1:240549/81‑1 (MQ=255) aaCAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCATTtga > 1:240566/1‑81 (MQ=255) tGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATc > 2:240573/1‑80 (MQ=255) aCGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCa < 2:240550/80‑1 (MQ=255) cATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGc > 2:240574/1‑80 (MQ=255) gTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAAc < 2:240551/80‑1 (MQ=255) tCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGa > 2:240575/1‑81 (MQ=255) tCGCCCTTGCGCATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCa > 2:240570/1‑81 (MQ=255) cTTGCGCATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTta > 2:240580/1‑80 (MQ=255) ttGCGCATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTtata > 1:240577/1‑81 (MQ=255) gcATAAGGGTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATAcgcg > 1:240576/1‑81 (MQ=255) gggTGCTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCAt > 1:240572/1‑80 (MQ=255) cTGATTTTTGTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATaacaac > 1:240578/1‑81 (MQ=255) | CGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATAACAAC > NC_000913/1349158‑1349301 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |