Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,428,030 C→G A233P (GCC→CCC)  insH‑5 ← Rac prophage; IS5 transposase and trans‑activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,428,0300CG96.3% 82.8 / NA 27A233P (GCC→CCC) insH‑5Rac prophage; IS5 transposase and trans‑activator
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (11/15);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTTGCGCGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGCCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCT  >  NC_000913/1427954‑1428091
                                                                            |                                                             
ttCTTGCGCGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAg                                                            <  2:251859/80‑1 (MQ=255)
      cgcgGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGctcctc                                                      <  2:251860/80‑1 (MQ=255)
       gcgGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTcgc                                                    <  2:251862/81‑1 (MQ=255)
       gcgGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTcgc                                                    <  2:251861/81‑1 (MQ=255)
           aTTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCtg                                                >  2:251889/1‑81 (MQ=255)
            ttCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCtgt                                               >  2:251906/1‑81 (MQ=255)
             tCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCtgtg                                              >  1:251896/1‑81 (MQ=255)
              cTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTgg                                             >  2:251894/1‑81 (MQ=255)
                 cTTCACCGTTTTTACCTTTCCTGGTCGCTCGCCGATCAGCCACTCCACATCCACCTCGGCCATCTCCTCACGCTGTGgcgc                                          >  2:251905/1‑81 (MQ=11)
                    cAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTcc                                       >  1:251898/1‑81 (MQ=255)
                         tttttACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGt                                  <  2:251863/81‑1 (MQ=255)
                          ttttACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTgg                                   >  2:251902/1‑79 (MQ=255)
                                    ccGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATc                        <  1:251864/80‑1 (MQ=255)
                                      gggACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGc                     >  2:251909/1‑81 (MQ=255)
                                       ggACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCt                    <  1:251865/81‑1 (MQ=255)
                                        gACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTg                   <  1:251866/81‑1 (MQ=37)
                                          cGCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTgag                 <  2:251867/81‑1 (MQ=18)
                                           gCTCGGCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTgaga                >  1:251910/1‑81 (MQ=18)
                                               ggCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACaaa            >  1:251901/1‑81 (MQ=255)
                                               ggCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACaaa            <  2:251869/81‑1 (MQ=255)
                                               ggCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACa              <  2:251868/79‑1 (MQ=255)
                                               ggCGATCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACa              <  1:251870/79‑1 (MQ=255)
                                                   aTCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGc        <  1:251871/81‑1 (MQ=18)
                                                    tCAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGct       <  2:251872/81‑1 (MQ=18)
                                                     cAGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGctc      >  2:251908/1‑81 (MQ=18)
                                                      aGCCAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGctcc     >  1:251897/1‑81 (MQ=18)
                                                         cAGTCCACATCCACCTCGGGCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCctct  <  1:251873/81‑1 (MQ=18)
                                                                            |                                                             
TTCTTGCGCGGATTCTGCTTCAAGGTTTTTACCTTGCCGGGACGCTCGGCGATCAGCCAGTCCACATCCACCTCGGCCAGCTCCTCGCGCTGTGGCGCTCCTTGGTAGCCGGCATCGGCTGAGACAAATTGCTCCTCT  >  NC_000913/1427954‑1428091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑