Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	164,352	C→T	L750L (CTG→TTG)	hrpB →	putative ATP‑dependent RNA helicase HrpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	164,352	0	C	T	100.0%	108.5 / NA	29	L750L (CTG→TTG)	hrpB	putative ATP‑dependent RNA helicase HrpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/15);  total (14/15)

CCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCG  >  NC_000913/164274‑164429                                                                               |                                                                              ccgcccgcGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTg                                                                             >  1:35138/1‑81 (MQ=255)    cccgcGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTg                                                                          <  2:35097/81‑1 (MQ=255)            ggCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGtt                                                                  >  2:35141/1‑81 (MQ=255)                gTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCtt                                                              <  2:35098/81‑1 (MQ=255)                 tGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCttt                                                             >  2:35151/1‑81 (MQ=255)                        gCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGc                                                      >  2:35163/1‑81 (MQ=255)                            gagaTGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCaa                                                  <  1:35099/81‑1 (MQ=255)                              gaTGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAg                                                >  2:35158/1‑81 (MQ=255)                               aTGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAgg                                               >  2:35157/1‑81 (MQ=255)                                 gTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGc                                              >  1:35150/1‑80 (MQ=255)                                  tttGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGcc                                             <  1:35100/80‑1 (MQ=255)                                  tttGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCa                                            >  1:35168/1‑81 (MQ=255)                                  tttGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCa                                            <  2:35101/81‑1 (MQ=255)                                   ttGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCAt                                           >  2:35162/1‑81 (MQ=255)                                    tGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCAt                                           <  1:35102/80‑1 (MQ=255)                                       cGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTAc                                        >  2:35153/1‑80 (MQ=255)                                           gCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAAt                                    >  1:35164/1‑80 (MQ=255)                                            ccaccaATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATca                                  <  2:35103/81‑1 (MQ=255)                                             caccaATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATca                                  >  1:35166/1‑80 (MQ=255)                                                caATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCacac                               >  1:35145/1‑80 (MQ=255)                                                caATCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCacac                               >  1:35159/1‑80 (MQ=255)                                                 aaTCCGACGATCGCCCAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACga                             <  1:35104/81‑1 (MQ=255)                                                               ccAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACtt               <  2:35105/81‑1 (MQ=255)                                                                cAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTc              <  2:35107/81‑1 (MQ=255)                                                                cAGGGGCGCGTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTc              <  2:35106/81‑1 (MQ=255)                                                                         gTGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGa     <  2:35108/81‑1 (MQ=255)                                                                          tGCCGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAg    <  1:35109/81‑1 (MQ=255)                                                                            ccGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGc   <  2:35110/80‑1 (MQ=255)                                                                            ccGTTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCg  <  1:35111/81‑1 (MQ=255)                                                                               |                                                                              CCGCCCGCGCTGGCGGTGAGAATGCAAGAGATGTTTGGCGAGGCCACCAATCCGACGATCGCCCAGGGGCGCGTGCCGCTGGTGCTGGAGTTGCTTTCACCTGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCG  >  NC_000913/164274‑164429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑