Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,546	A→C	Y100D (TAT→GAT)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,546	0	A	C	100.0%	74.8 / NA	21	Y100D (TAT→GAT)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/15);  total (6/15)

CTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGC  >  NC_000913/1442469‑1442622                                                                              |                                                                             cTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGc                                                                           <  1:253797/81‑1 (MQ=255)    aTACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTg                                                                         <  1:253798/80‑1 (MQ=255)       cgcgcgGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAac                                                                     <  2:795293/81‑1 (MQ=255)       cgcgcgGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAac                                                                     <  1:253799/81‑1 (MQ=255)           cgGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACgg                                                                 <  2:253800/81‑1 (MQ=255)                  tACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACtt                                                          <  1:253801/81‑1 (MQ=255)                         gTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGccc                                                   <  1:253802/81‑1 (MQ=255)                         gTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGccc                                                   >  1:253832/1‑81 (MQ=255)                                   atcatcatACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCtttt                                         <  2:253803/81‑1 (MQ=255)                                       tcatACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCcg                                     <  2:253804/81‑1 (MQ=255)                                                tGGCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGt                            <  2:253805/81‑1 (MQ=255)                                                 ggCGTGTTCAGCAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTc                           >  1:253845/1‑81 (MQ=255)                                                            cAACGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTg                 >  1:253848/1‑80 (MQ=255)                                                              aCGGCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGaaa              <  2:253806/81‑1 (MQ=255)                                                                ggCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAAcc            >  2:253831/1‑81 (MQ=255)                                                                ggCCTCTGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAAcc            <  1:253807/81‑1 (MQ=255)                                                                   ctctGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGc         <  2:253808/81‑1 (MQ=255)                                                                     ctGGATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGca        >  2:253837/1‑80 (MQ=255)                                                                       ggATCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAg     >  2:253826/1‑81 (MQ=255)                                                                          tCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAgcgc  <  2:253809/81‑1 (MQ=255)                                                                          tCATCGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAgcgc  <  2:253810/81‑1 (MQ=255)                                                                              |                                                                             CTGATACGCGCGGTGAATACGGCGGTTCAGCGTCATCATCATACCGATGGCGTGTTCAGCAACGGCCTCTGGATCATAGGCTGGAACACGGACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGC  >  NC_000913/1442469‑1442622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑