Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,760	A→C	D302A (GAT→GCT)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,760	0	A	C	100.0%	74.1 / NA	21	D302A (GAT→GCT)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/16);  total (5/16)

GATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATT  >  NC_000913/1497683‑1497826                                                                              |                                                                   gATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCg                                                                 >  1:261544/1‑81 (MQ=255)   tGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTc                                                               <  1:261513/81‑1 (MQ=255)     aCCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCt                                                              <  2:261514/80‑1 (MQ=255)         gTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCa                                                         <  2:261515/81‑1 (MQ=255)                      cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGc                                              <  1:261516/79‑1 (MQ=255)                       ggTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAAc                                           <  2:261517/81‑1 (MQ=255)                        gTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACg                                          <  2:261518/81‑1 (MQ=255)                             aaTGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAg                                     >  2:261547/1‑81 (MQ=255)                              aTGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAg                                     <  2:261520/80‑1 (MQ=255)                              aTGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGt                                    <  2:261519/81‑1 (MQ=255)                                    gTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGAttt                              <  1:261521/81‑1 (MQ=255)                                      cGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGc                            >  2:261545/1‑81 (MQ=255)                                         aTGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGt                         <  2:261522/81‑1 (MQ=255)                                            tgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTcc                       <  2:261523/80‑1 (MQ=255)                                              cGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCt                    <  2:261524/81‑1 (MQ=255)                                               gATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTg                   <  2:261525/81‑1 (MQ=255)                                                 tACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGaa                 <  2:261526/81‑1 (MQ=255)                                                   cAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGaat                <  1:261527/80‑1 (MQ=255)                                                        cATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATcc           <  2:261528/80‑1 (MQ=255)                                                           ccGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCa        >  2:261546/1‑80 (MQ=255)                                                                aaTTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAttt  >  2:261541/1‑81 (MQ=255)                                                                              |                                                                   GATGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATT  >  NC_000913/1497683‑1497826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑