Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,572,565	T→C	T880A (ACT→GCT)	pqqL ←	putative zinc peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,572,565	0	T	C	100.0%	34.0 / NA	11	T880A (ACT→GCT)	pqqL	putative zinc peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/4);  total (7/4)

TCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCAT  >  NC_000913/1572510‑1572633                                                        |                                                                     tCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAacgctacgc                                             >  1:271560/1‑81 (MQ=255)  ccTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAacgctacgct                                            >  1:271559/1‑81 (MQ=255)      cTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTg                                        <  2:271545/81‑1 (MQ=255)        cAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGa                                      >  1:271558/1‑81 (MQ=255)        cAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGa                                      >  2:271546/1‑81 (MQ=255)                    cAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCt                            <  1:271548/79‑1 (MQ=255)                    cAGGATCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTg                           <  2:271547/80‑1 (MQ=255)                        aTCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCt                       >  2:271557/1‑80 (MQ=255)                        aTCGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTg                      <  1:271549/81‑1 (MQ=255)                          cGTCATATTGAATAAGACTATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGaa                    >  1:271565/1‑81 (MQ=255)                                            tATTTACAATAGCGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTattcat  >  1:271566/1‑81 (MQ=255)                                                        |                                                                     TCCTGCTCAGTCCATGCTGCAGGATCGTCATATTGAATAAGACTATTTACAATAGTGTTCGCTAATTGTTGAACGCTACGCTGTTGGATATCGAGGCTGCGCTGAACGTTTTGCTGGTATTCAT  >  NC_000913/1572510‑1572633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑