Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,607,828	C→T	G161G (GGC→GGT)	tam →	trans‑aconitate 2‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,607,828	0	C	T	100.0%	85.4 / NA	23	G161G (GGC→GGT)	tam	trans‑aconitate 2‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/10);  total (13/10)

GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACC  >  NC_000913/1607750‑1607891                                                                               |                                                                ggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGgtgt                                                               >  2:275870/1‑81 (MQ=255)  gAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGtt                                                              <  2:275843/81‑1 (MQ=255)   aGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGtt                                                              >  2:275882/1‑80 (MQ=255)      cGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATg                                                          >  1:275880/1‑81 (MQ=255)       gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGc                                                         >  1:275883/1‑81 (MQ=255)              gtgCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTtactac                                                  >  1:275892/1‑81 (MQ=255)                    aTGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAtt                                            >  2:275889/1‑81 (MQ=255)                     tGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAttt                                           <  2:275844/81‑1 (MQ=255)                        gcgAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATAtttt                                          >  2:275875/1‑79 (MQ=255)                                  cTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCg                               >  2:275887/1‑80 (MQ=255)                                        aCAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGAtgt                          >  2:275879/1‑79 (MQ=255)                                         cAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAg                       <  2:275845/81‑1 (MQ=255)                                             aCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCg                   <  1:275846/81‑1 (MQ=255)                                                   cGGATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATCTTGAGCGAAGCCGGATGTGAGGTCGATATCt             >  2:275888/1‑81 (MQ=255)                                                    ggATCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTAAGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTg            >  2:275904/1‑81 (MQ=255)                                                      aTCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGc          <  1:275847/81‑1 (MQ=255)                                                      aTCGCGGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGc          >  1:275885/1‑81 (MQ=255)                                                         gcgGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGaa       <  2:275849/81‑1 (MQ=255)                                                         gcgGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGa        <  2:275848/80‑1 (MQ=255)                                                          cgGGCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac      <  2:275850/81‑1 (MQ=255)                                                           gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAacc     <  2:275851/81‑1 (MQ=255)                                                           gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac      >  1:275894/1‑80 (MQ=255)                                                           gggCGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAac      >  2:275898/1‑80 (MQ=255)                                                              cGTGAGCCGTTGGCTGGTGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAaccacc  <  2:275852/81‑1 (MQ=255)                                                                               |                                                                GGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACC  >  NC_000913/1607750‑1607891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑