Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,609,077	C→T	intergenic (‑55/+152)	yneE ← / ← uxaB	conserved inner membrane protein YneE/tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,609,077	0	C	T	100.0%	68.5 / NA	19	intergenic (‑55/+152)	yneE/uxaB	conserved inner membrane protein YneE/tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/13);  total (6/13)

TGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAA  >  NC_000913/1609008‑1609153                                                                      |                                                                             tgGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTa                                                                   <  1:276039/81‑1 (MQ=255)   gACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTAc                                                                  <  1:276040/80‑1 (MQ=255)    aCGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACtt                                                                <  2:276041/81‑1 (MQ=255)    aCGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACtt                                                                <  2:276042/81‑1 (MQ=255)     cGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTa                                                               >  1:276065/1‑81 (MQ=255)      gAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTAc                                                              <  2:276043/81‑1 (MQ=255)        aCAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACa                                                             >  1:276068/1‑80 (MQ=255)         cAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACaaa                                                           <  2:276044/81‑1 (MQ=255)               tGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCt                                                     <  1:276045/81‑1 (MQ=255)                           ggCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTacaa                                         <  1:276046/81‑1 (MQ=255)                                  aaaaGTCGCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAg                                   >  1:276067/1‑80 (MQ=255)                                         gCCCTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCt                           >  2:276075/1‑81 (MQ=255)                                           ccTATTAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTg                          >  1:276066/1‑80 (MQ=255)                                               ttAAACGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAtg                      <  2:276047/80‑1 (MQ=255)                                                 aaaCGTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCAtgtg                    <  1:276048/80‑1 (MQ=255)                                                     gTGATTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCAcc               >  2:276071/1‑81 (MQ=255)                                                        aTTACGATCACATTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTg             <  2:276049/80‑1 (MQ=255)                                                                 acaTTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATaa   <  2:276050/81‑1 (MQ=255)                                                                  caTTTTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATaaa  <  2:276051/81‑1 (MQ=255)                                                                      |                                                                             TGGACGAACAATCATGGTTATCTTCAGGCGTGGAAAAGTCGCCCTATTAAACGTGATTACGATCACATTCTCAAGACGCTACTTACAAATTACCTACAAGCTTACAACATTAGCAGGCGCTGCATGTGGCACCTGTTAATGATAAA  >  NC_000913/1609008‑1609153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑