Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,589	A→G	V31A (GTT→GCT)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,589	0	A	G	100.0%	74.2 / NA	21	V31A (GTT→GCT)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

GTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGA  >  NC_000913/1610514‑1610666                                                                            |                                                                              gTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTTATTCAGGAGATCGATTTGCCAGTCAGCAAAg                                                                          >  2:276271/1‑81 (MQ=255)       aTCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgcgcgc                                                                    <  1:276243/81‑1 (MQ=255)       aTCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgcgcgc                                                                    <  2:276242/81‑1 (MQ=255)        tCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGgcgcg                                                                     >  1:276268/1‑79 (MQ=255)           gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAgg                                                                 >  2:276270/1‑80 (MQ=255)           gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGa                                                                <  1:276244/81‑1 (MQ=255)                   acGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCt                                                        <  1:276245/81‑1 (MQ=255)                     gACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCtt                                                       >  2:276273/1‑80 (MQ=255)                      aCCACGCCAGAATTTAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCa                                                     >  2:276269/1‑81 (MQ=255)                      aCCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCa                                                     <  1:276246/81‑1 (MQ=255)                             cAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACt                                              >  2:276265/1‑81 (MQ=255)                                     aGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATa                                      >  2:276281/1‑81 (MQ=255)                                          ggTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTc                                 <  1:276247/81‑1 (MQ=255)                                                   aTTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACtg                        <  1:276248/81‑1 (MQ=255)                                                     tCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACtgtg                      >  1:276282/1‑81 (MQ=255)                                                                aTTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGagaa           >  2:276277/1‑81 (MQ=255)                                                                     ccAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgcg      <  2:276249/81‑1 (MQ=255)                                                                      cAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGcga     >  2:276285/1‑81 (MQ=255)                                                                       aGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGcgac    <  2:276251/81‑1 (MQ=255)                                                                       aGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGcgac    <  1:276250/81‑1 (MQ=255)                                                                         tCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGcgacga  <  2:276252/81‑1 (MQ=255)                                                                            |                                                                              GTTTCAATCGGACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCGACGA  >  NC_000913/1610514‑1610666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑