Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,658,706	G→C	G213A (GGC→GCC)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,658,706	0	G	C	100.0%	85.7 / NA	24	G213A (GGC→GCC)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/12);  total (12/12)

TCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658630‑1658783                                                                             |                                                                              tCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCaa                                                                            >  1:281790/1‑80 (MQ=255)  cACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAAc                                                                           <  1:281764/80‑1 (MQ=255)    cTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGc                                                                        <  2:281765/81‑1 (MQ=255)       aaCCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGcacc                                                                     <  2:281766/81‑1 (MQ=255)       aaCCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGcac                                                                      >  2:281810/1‑80 (MQ=255)       aaCCAGTATGGCTCCTACAGCACTGCGCAAAATTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGcacc                                                                     <  2:281767/81‑1 (MQ=255)                    ccTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaa                                                         >  2:281798/1‑80 (MQ=255)                     cTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaa                                                         <  2:281768/79‑1 (MQ=255)                      tACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGaaaa                                                       >  2:281808/1‑80 (MQ=255)                          gCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGc                                                   >  2:281811/1‑80 (MQ=255)                                 gcAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGt                                           >  2:281802/1‑81 (MQ=255)                                  cAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCCGGTc                                          <  2:281769/81‑1 (MQ=255)                                             tgCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTgg                               <  1:281771/81‑1 (MQ=255)                                             tgCCATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTg                                <  1:281770/80‑1 (MQ=255)                                               ccATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGt                              <  1:281772/80‑1 (MQ=255)                                                cATGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTaa                            <  1:281773/81‑1 (MQ=255)                                                 aTGCCCTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTaac                           <  1:281774/81‑1 (MQ=255)                                                     ccTACACCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAAcc                       >  1:281800/1‑81 (MQ=255)                                                          acCTACGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCgg                    >  2:281803/1‑79 (MQ=255)                                                              aCGGCAGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGaaa              >  2:281813/1‑81 (MQ=255)                                                                   aGTAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCg          >  2:281806/1‑80 (MQ=255)                                                                     tAATGATGCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCAt       >  1:281814/1‑81 (MQ=255)                                                                          atgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGc   <  1:281775/80‑1 (MQ=255)                                                                          atgCCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCg  >  1:281809/1‑81 (MQ=255)                                                                             |                                                                              TCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATGATGGCAACAGCACCACGGATATTGAAAACAGCAAGCTGGTCGTGATGTTTGGTAACAACCCGGCAGAAACCCGCATGAGCG  >  NC_000913/1658630‑1658783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑