Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,774,321	T→C	V178A (GTC→GCC)	ydiB →	shikimate dehydrogenase/quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,774,321	0	T	C	100.0%	61.8 / NA	18	V178A (GTC→GCC)	ydiB	shikimate dehydrogenase/quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/5);  total (13/5)

AACTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTT  >  NC_000913/1774243‑1774386                                                                               |                                                                  aaCTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTgccg                                                                 <  2:297479/81‑1 (MQ=255)    tctTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCa                                                              >  2:297513/1‑81 (MQ=255)           ccGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCAc                                                       >  1:297519/1‑81 (MQ=255)                gggATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGAtc                                                  <  1:297481/81‑1 (MQ=255)                   aTGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCg                                               <  1:297482/81‑1 (MQ=255)                       gTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGa                                           >  2:297507/1‑81 (MQ=255)                          cttcGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGAt                                          >  2:297512/1‑79 (MQ=255)                                          gCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTg                          >  1:297516/1‑79 (MQ=255)                                          gCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTg                          >  2:297509/1‑79 (MQ=255)                                           ccTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAg                       <  2:297483/81‑1 (MQ=255)                                                 cgcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTgg                 >  1:297514/1‑81 (MQ=255)                                                  gcAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGc                >  2:297527/1‑81 (MQ=255)                                                    aGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCtt              >  2:297524/1‑81 (MQ=255)                                                       gggTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccg           >  2:297508/1‑81 (MQ=255)                                                       gggTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccg           >  2:297526/1‑81 (MQ=255)                                                           tAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGa       >  2:297530/1‑81 (MQ=255)                                                            aaTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGa       >  2:297518/1‑80 (MQ=255)                                                             aTGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGAc      <  2:297484/80‑1 (MQ=255)                                                                aaaaCACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACAttt  <  1:297485/81‑1 (MQ=255)                                                                               |                                                                  AACTCTTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTT  >  NC_000913/1774243‑1774386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑