Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,866	T→C	Y360C (TAT→TGT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,866	0	T	C	100.0%	116.7 / NA	32	Y360C (TAT→TGT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/12);  total (20/12)

AGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796795‑1796938                                                                        |                                                                         aGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTgag                                                                 <  2:300672/81‑1 (MQ=255)   aTGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGAc                                                               >  1:300716/1‑81 (MQ=255)      tCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCa                                                            <  2:300673/81‑1 (MQ=255)        gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                          <  1:300674/81‑1 (MQ=255)        gATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATc                                                          >  1:300730/1‑81 (MQ=255)            agcagACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTa                                                      <  1:300675/81‑1 (MQ=255)                  cGGGTCGCACGTTCCATCGCTTTGTGTTGCAGTGCCGGATCAACGCCACGCCCACAACGGTGAGACGCATCGGTATGCAgg                                                >  1:300710/1‑81 (MQ=255)                     gTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCa                                             >  1:300711/1‑81 (MQ=255)                          aCGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                         >  2:300740/1‑80 (MQ=255)                          aCGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                         >  1:300725/1‑80 (MQ=255)                             ttCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgacgac                                      >  1:300726/1‑80 (MQ=255)                             ttCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgacgac                                      >  1:300717/1‑80 (MQ=255)                             ttCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGacgacg                                     <  1:300676/81‑1 (MQ=255)                               ccATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAg                                   >  1:300723/1‑81 (MQ=255)                                  tCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCAc                                <  1:300677/81‑1 (MQ=255)                                  tCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCAc                                >  2:300720/1‑81 (MQ=255)                                   cGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCAc                                >  2:300732/1‑80 (MQ=255)                                      tttGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGAcc                            >  2:300719/1‑81 (MQ=255)                                          tgctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGt                         <  1:300678/80‑1 (MQ=255)                                          tgctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGt                         <  2:300679/80‑1 (MQ=255)                                           gctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGa                       >  2:300729/1‑81 (MQ=255)                                               cAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAg                    <  1:300680/80‑1 (MQ=255)                                                   gCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGc               >  2:300728/1‑81 (MQ=255)                                                    ccGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCg              <  2:300681/81‑1 (MQ=255)                                                    ccGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCg              >  2:300727/1‑81 (MQ=255)                                                      ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg            >  1:300724/1‑81 (MQ=255)                                                      ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg            >  2:300731/1‑81 (MQ=255)                                                           aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGATGAGCACGACCGGTGATAGACAGCGGGCTa         >  2:300742/1‑79 (MQ=255)                                                             cGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGa     <  1:300682/81‑1 (MQ=255)                                                              gCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGaa    >  1:300713/1‑81 (MQ=255)                                                               ccACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAc   <  2:300683/81‑1 (MQ=255)                                                                cACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcg  >  1:300737/1‑81 (MQ=255)                                                                        |                                                                         AGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACG  >  NC_000913/1796795‑1796938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑