Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,943	T→C	Y347C (TAC→TGC)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,943	0	T	C	100.0%	105.9 / NA	29	Y347C (TAC→TGC)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/19);  total (10/19)

CACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAT  >  NC_000913/1830867‑1831019                                                                             |                                                                             cacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATc                                                                          <  2:305213/81‑1 (MQ=255)  acGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCg                                                                         >  2:305256/1‑81 (MQ=255)    gTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGc                                                                       <  2:305214/81‑1 (MQ=255)      tGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCAt                                                                     >  2:305215/1‑81 (MQ=255)        ccACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTc                                                                   <  1:305216/81‑1 (MQ=255)         cACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCa                                                                  <  1:305217/81‑1 (MQ=255)              gCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTaca                                                             <  2:305218/81‑1 (MQ=255)               cAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTaca                                                             >  2:305264/1‑80 (MQ=255)                 aTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAg                                                          >  2:305258/1‑81 (MQ=255)                   cAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGc                                                         >  1:305255/1‑80 (MQ=255)                     gTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCaa                                                      <  1:305219/81‑1 (MQ=255)                         cATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAg                                                  >  1:305261/1‑81 (MQ=255)                           tCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCa                                                <  2:305220/81‑1 (MQ=255)                              cGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAAc                                             <  2:305222/81‑1 (MQ=255)                              cGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAAc                                             <  1:305221/81‑1 (MQ=255)                                 cTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCt                                           >  1:305260/1‑80 (MQ=255)                                    cTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAg                                        <  1:305224/80‑1 (MQ=255)                                    cTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGc                                       <  1:305223/81‑1 (MQ=255)                                         gccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgcg                                   >  1:305263/1‑80 (MQ=255)                                         accgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGa                                  <  1:741850/80‑1 (MQ=255)                                           cgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAAc                                <  1:305225/81‑1 (MQ=255)                                            gcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACt                               >  2:305247/1‑81 (MQ=255)                                                             tttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCAt               <  2:305226/80‑1 (MQ=255)                                                             tttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCAt               <  1:305227/80‑1 (MQ=255)                                                                cGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGc            <  1:305228/80‑1 (MQ=255)                                                                     gCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTg        <  2:305229/79‑1 (MQ=255)                                                                      cccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAtt     <  2:305230/81‑1 (MQ=255)                                                                      cccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAt      >  2:305265/1‑80 (MQ=255)                                                                         ggCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAt  <  1:305231/81‑1 (MQ=255)                                                                             |                                                                             CACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAAT  >  NC_000913/1830867‑1831019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑