Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,899,060	A→C	N212H (AAT→CAT)	pdeD →	putative c‑di‑GMP phosphodiesterase PdeD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,899,060	0	A	C	100.0%	73.9 / NA	21	N212H (AAT→CAT)	pdeD	putative c‑di‑GMP phosphodiesterase PdeD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/12);  total (9/12)

CGCCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACAATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGGCGCTGG  >  NC_000913/1898984‑1899135                                                                             |                                                                            cgcCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATg                                                                          >  2:314791/1‑80 (MQ=255) cgcCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGa                                                                         <  2:314770/81‑1 (MQ=255)  gcCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGaa                                                                        <  2:314771/81‑1 (MQ=255)        cTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTAt                                                                  >  2:314815/1‑81 (MQ=255)                 gACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTttc                                                          <  2:314772/80‑1 (MQ=255)                        ggCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAAc                                                  >  1:314807/1‑81 (MQ=255)                         gCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACg                                                 >  2:314793/1‑81 (MQ=255)                          cATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGc                                                <  1:314773/81‑1 (MQ=255)                               gCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTc                                            <  2:314774/80‑1 (MQ=255)                                        tAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCAt                                  <  1:314775/81‑1 (MQ=255)                                           tGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTaa                               <  2:314776/81‑1 (MQ=255)                                           tGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTaa                               >  2:314806/1‑81 (MQ=255)                                                  gTAGATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAAt                        <  1:314777/81‑1 (MQ=255)                                                     gATTCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGt                     >  1:314799/1‑81 (MQ=255)                                                       ttCCCTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTcc                   <  2:314778/81‑1 (MQ=255)                                                           cTTCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCgggg               >  1:314800/1‑81 (MQ=255)                                                             tCCGCAACCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGc             >  1:314809/1‑81 (MQ=255)                                                                   aCCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGgc       <  2:314779/81‑1 (MQ=255)                                                                   aCCTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGgc       <  2:314780/81‑1 (MQ=255)                                                                     cTGAAGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGgcgc     <  1:314781/81‑1 (MQ=255)                                                                         aGACCATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGGCGCtgg  >  2:314795/1‑80 (MQ=255)                                                                             |                                                                            CGCCAGCCTGACGGTGGACAAACGGCATTTGCTCTATGGTAATGGGCTGGTAGATTCCCTTCCGCAACCTGAAGACAATGAAAACTATCAGGTTTCTTCGCAACGCTTTCCTTTTACCATTAACGTTAATGGTCCGGGGGCTACGGCGCTGG  >  NC_000913/1898984‑1899135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑