Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,485	A→G	E210G (GAA→GGA)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,485	0	A	G	100.0%	133.8 / NA	37	E210G (GAA→GGA)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

AAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTA  >  NC_000913/191407‑191562                                                                               |                                                                              aaGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGAGATGCAGTCTGGTAAGCCgaaaggaa                                                                             <  1:41132/81‑1 (MQ=255)    aCGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                          >  1:41195/1‑81 (MQ=255)    aCGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                          <  2:41133/81‑1 (MQ=255)      gTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCa                                                                        <  1:41134/81‑1 (MQ=255)        aTCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                          >  1:41178/1‑77 (MQ=255)                aaGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATgg                                                              >  1:41184/1‑81 (MQ=255)                 aGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGt                                                             <  2:41137/81‑1 (MQ=255)                 aGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGt                                                             <  2:41136/81‑1 (MQ=255)                 aGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGt                                                             <  1:41135/81‑1 (MQ=255)                      tggAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAg                                                        <  1:41138/79‑1 (MQ=255)                      tAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAg                                                        >  2:41183/1‑81 (MQ=255)                        gAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGc                                                      <  1:41139/81‑1 (MQ=255)                        gAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGc                                                      >  2:41173/1‑81 (MQ=255)                         aaaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGcc                                                     <  1:41140/81‑1 (MQ=255)                                aTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                                >  1:41182/1‑79 (MQ=255)                                  aCCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaag                                             >  1:41192/1‑80 (MQ=255)                                   ccAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaaga                                            >  1:41194/1‑80 (MQ=255)                                    cAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGaaa                                          >  1:41190/1‑81 (MQ=255)                                    cAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGaaa                                          >  1:41187/1‑81 (MQ=255)                                      ggTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAAtt                                        >  1:41185/1‑81 (MQ=255)                                         aCAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAc                                     >  2:41191/1‑81 (MQ=255)                                           aGCTGGATATCGCGATGCAGTCTGGTAAGCTGAAAGGAATCGCAGAGAATATGGTTGAAGGCCGCATGAAGAAATTCACCg                                   >  1:41179/1‑81 (MQ=255)                                              tGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGc                                 >  1:41189/1‑80 (MQ=255)                                                gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCg                                <  1:41142/79‑1 (MQ=255)                                                gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGaa                              <  2:41143/81‑1 (MQ=255)                                                gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGa                               <  2:41141/80‑1 (MQ=255)                                                 atatCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAg                             >  2:41186/1‑81 (MQ=255)                                                 atatCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAACGGCGAAg                             >  1:41202/1‑81 (MQ=255)                                                      gcgATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTtc                         <  1:41144/80‑1 (MQ=255)                                                       cgATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTtctc                       >  1:41197/1‑81 (MQ=255)                                                        gATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTctct                      >  1:41218/1‑81 (MQ=255)                                                          tGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGa                    <  1:41145/81‑1 (MQ=255)                                                                   gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTc              <  2:41147/78‑1 (MQ=255)                                                                   gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCa             <  1:41146/79‑1 (MQ=255)                                                                     aaGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcg         >  2:41210/1‑81 (MQ=255)                                                                      aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgt        <  2:41148/81‑1 (MQ=255)                                                                       gCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt       <  1:41149/81‑1 (MQ=255)                                                                        ccGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttc      <  1:41150/81‑1 (MQ=255)                                                                            aaggaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTa  >  2:41201/1‑81 (MQ=255)                                                                               |                                                                              AAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTA  >  NC_000913/191407‑191562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑