Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,967,269	C→A	D57Y (GAC→TAC)	cheY ←	chemotaxis protein CheY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,967,269	0	C	A	100.0%	93.7 / NA	25	D57Y (GAC→TAC)	cheY	chemotaxis protein CheY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/12);  total (13/12)

CCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTA  >  NC_000913/1967200‑1967345                                                                      |                                                                             ccGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACa                                                                    <  2:324916/80‑1 (MQ=255)           gcCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAAcc                                                         <  1:324917/81‑1 (MQ=255)            ccATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCg                                                        >  2:324947/1‑81 (MQ=255)                ccGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCgcctg                                                    >  1:324918/1‑81 (MQ=255)                 cGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCgcctgc                                                   <  1:324919/81‑1 (MQ=255)                  gCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCgcctgcc                                                  <  1:324920/81‑1 (MQ=255)                   cACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCgcctgcct                                                 >  1:324955/1‑81 (MQ=255)                      gAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCctgcctgc                                               >  1:324949/1‑80 (MQ=255)                      gAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCctgcctgc                                               >  2:324946/1‑80 (MQ=255)                               tCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTAtt                                       >  2:324951/1‑79 (MQ=255)                                 aGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTgaga                                   <  1:324921/81‑1 (MQ=255)                                      ttCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTc                              >  1:324945/1‑81 (MQ=255)                                      ttCCAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTc                              <  1:324922/81‑1 (MQ=255)                                        ccAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGa                            >  1:324964/1‑81 (MQ=255)                                         cAGGCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGAc                           <  2:324923/81‑1 (MQ=255)                                            gCCATCCATATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGcc                        <  1:324924/81‑1 (MQ=255)                                              catccatATTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCAt                      >  2:324953/1‑81 (MQ=255)                                                   atatTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTcc                 <  1:324925/81‑1 (MQ=255)                                                    tatTGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCg                <  1:324926/81‑1 (MQ=255)                                                      ttGGGCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGc               <  1:324927/80‑1 (MQ=255)                                                         ggCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTcc           >  1:324957/1‑81 (MQ=255)                                                         ggCATGTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTcc           <  2:324928/81‑1 (MQ=255)                                                              gTTCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCaa       >  1:324962/1‑80 (MQ=255)                                                                tCCAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACat    >  2:324966/1‑81 (MQ=255)                                                                  cAGTAGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACatta  >  1:324948/1‑81 (MQ=255)                                                                      |                                                                             CCGACATCGCGCCATCCGCACGAATTGTTTTCAGCAATTCCAGGCCATCCATATTGGGCATGTTCCAGTCGGAGATAACAAATCCATAACCGCCTGCCTGCAACTTATTGAGAGCGTCGACGCCATCTTCCGCTTCCTCAACATTA  >  NC_000913/1967200‑1967345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑