| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,977,712 | T→C | K45E (AAA→GAA) | flhC ← | DNA‑binding transcriptional dual regulator FlhC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,977,712 | 0 | T | C | 88.2% | 42.9 / ‑0.1 | 17 | K45E (AAA→GAA) | flhC | DNA‑binding transcriptional dual regulator FlhC |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (4/11); total (4/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGAT > NC_000913/1977634‑1977787 | cGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTtat < 1:326625/81‑1 (MQ=255) ttttGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATaa < 1:326626/81‑1 (MQ=255) cccAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGtttt < 1:326627/80‑1 (MQ=255) ggTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAg < 2:326628/81‑1 (MQ=255) tCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGc < 1:326629/81‑1 (MQ=255) aaaCCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTcc > 2:326661/1‑81 (MQ=255) aaCCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTcc < 2:326630/80‑1 (MQ=255) aCCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCcgc < 2:326631/81‑1 (MQ=255) gTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACt < 2:326632/81‑1 (MQ=255) tCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACtt < 2:326633/81‑1 (MQ=255) gaATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTgtgt < 2:326634/81‑1 (MQ=255) gCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAg > 2:326659/1‑81 (MQ=255) cTTTCGGCGGTGGGCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCa > 1:326670/1‑81 (MQ=255) gCTTCCGCGCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAgc < 1:326635/81‑1 (MQ=255) cgcgCAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCa < 1:326636/81‑1 (MQ=255) gcgcAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAg < 1:326637/81‑1 (MQ=255) gcAGTTCTTCATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAggg > 2:326672/1‑81 (MQ=255) cAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGt < 1:326638/81‑1 (MQ=255) ttcttcATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGAt < 2:326639/81‑1 (MQ=255) | CGTTTTGTTCCCAGGTCATAAACCAGTCGGTTGAGAATGGCAGCATGCCTTTCGGCGGTGGGCTTCCGCGCAGTTCTTTATAAAGTTTTATCAGGCGTCCGCGACTTAACTGTGTTTCGCTTTCCAGCATCTGCAAACGAGCGCCCAGGGTGAT > NC_000913/1977634‑1977787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |