Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,990,885	T→A	V402E (GTG→GAG)	tyrP →	tyrosine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,990,885	0	T	A	100.0%	78.3 / NA	21	V402E (GTG→GAG)	tyrP	tyrosine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/8);  total (13/8)

GTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAG  >  NC_000913/1990812‑1990962                                                                          |                                                                              gtggtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTg                                                                         <  2:328452/80‑1 (MQ=255)    gtggtgTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCa                                                                     <  2:328453/81‑1 (MQ=255)            tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGcc                                                              <  1:328454/80‑1 (MQ=255)            tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCt                                                             >  1:328471/1‑81 (MQ=255)            tctcTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCt                                                             >  1:328477/1‑81 (MQ=255)             ctctGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCt                                                             >  2:328472/1‑80 (MQ=255)                  tgGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCaa                                                          <  1:328455/78‑1 (MQ=255)                    gTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTcc                                                     <  2:328456/81‑1 (MQ=255)                                     gTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAAt                                    >  2:328476/1‑81 (MQ=255)                                      tGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAAtt                                   <  2:328457/81‑1 (MQ=255)                                         aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                >  1:328482/1‑81 (MQ=255)                                         aaTTTTTGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAAc                                >  1:328467/1‑81 (MQ=255)                                              ttGATTGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTg                            <  1:328458/80‑1 (MQ=255)                                                  ttGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAcc                       <  2:328459/81‑1 (MQ=255)                                                   tGCGGCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACAcc                       >  1:328474/1‑80 (MQ=255)                                                       gCAGGGTTGTTACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAAtt                  >  1:328486/1‑81 (MQ=255)                                                               gttACCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCctct          >  1:328487/1‑81 (MQ=255)                                                                  aCCAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTaa       >  2:328480/1‑81 (MQ=255)                                                                    cAGAAGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATg     >  2:328481/1‑81 (MQ=255)                                                                       aaGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag  >  1:328488/1‑81 (MQ=255)                                                                       aaGAGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATgcag  >  1:328483/1‑81 (MQ=255)                                                                          |                                                                              GTGGTGGTGTTTCTCTGTGGTATTGCTGTGATTGGCGTGCAATTTTTGATTGCGGCAGGGTTGTTACCAGAAGTGGGGTGATCAGATAGCCTCAAATTCCTTATTGGGTGCCAGAATTAACGCTGACACCCAATTTGGCCTCTTAATGCAG  >  NC_000913/1990812‑1990962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑