Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,681	T→C	Y9Y (TAT→TAC)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,681	0	T	C	100.0%	47.9 / NA	14	Y9Y (TAT→TAC)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/10);  total (4/10)

AATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTA  >  NC_000913/1995609‑1995756                                                                         |                                                                            aaTATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACg                                                                      <  1:329130/80‑1 (MQ=255)   tatGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTAt                                                                   <  2:329131/81‑1 (MQ=255)          cATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAg                                                            >  2:329145/1‑81 (MQ=255)           attaattaACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGc                                                           <  1:329132/81‑1 (MQ=255)                   aCCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCg                                                    <  2:329133/80‑1 (MQ=255)                      cGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGatat                                                <  2:329134/81‑1 (MQ=255)                             tACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCt                                          >  1:329147/1‑80 (MQ=255)                             tACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTc                                         <  1:329135/81‑1 (MQ=255)                             tACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTc                                         <  1:329136/81‑1 (MQ=255)                                    aaaaaaCATTCATGATAAAAATATTTATTGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAaac                                  <  1:329137/81‑1 (MQ=255)                                                             tattGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAgg          <  1:329138/80‑2 (MQ=255)                                                              attGGTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAggt         >  2:329157/1‑78 (MQ=255)                                                                 ggTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt      <  1:329139/80‑1 (MQ=255)                                                                  gTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATgg    >  2:329149/1‑81 (MQ=255)                                                                    cattacattaACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGta  >  1:329151/1‑81 (MQ=255)                                                                         |                                                                            AATATGTAACATTAATTAACCCGAACAATACGGGCAAAAAACATTCATGATAAAAATATTTATTGGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTA  >  NC_000913/1995609‑1995756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑