Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,995,748	C→G	L32V (CTT→GTT)	yecU →	protein YecU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,995,748	0	C	G	100.0%	59.3 / NA	17	L32V (CTT→GTT)	yecU	protein YecU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (12/5);  total (12/5)

GGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAA  >  NC_000913/1995673‑1995817                                                                            |                                                                      ggTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTAt                                                                   <  1:329139/80‑1 (MQ=255)  gTCATTACATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATgg                                                                 >  2:329149/1‑81 (MQ=255)    cattacattaACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGta                                                               >  1:329151/1‑81 (MQ=255)            tAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTccgc                                                       >  2:329150/1‑81 (MQ=255)              aCGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTccgcc                                                      <  2:329140/80‑1 (MQ=255)                         aGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACAttt                                          >  2:329156/1‑81 (MQ=255)                               gCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCAcc                                    >  1:329161/1‑81 (MQ=255)                                  gATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGc                                  >  1:329154/1‑80 (MQ=255)                                  gATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGc                                  >  2:329153/1‑80 (MQ=255)                                    tatCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCaaa                               <  2:329141/81‑1 (MQ=255)                                                 aaaCAACCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCaa                   >  2:329142/1‑80 (MQ=255)                                                    caacCACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACAt                >  2:329155/1‑80 (MQ=255)                                                       ccACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGAc             >  2:329159/1‑80 (MQ=255)                                                        cACTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGa           <  1:329143/81‑1 (MQ=255)                                                         aCTGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAg          >  1:329158/1‑81 (MQ=255)                                                           tGCTATTTTTAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAgg         >  2:329160/1‑80 (MQ=255)                                                                  tttAGCAAAGGTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAaaataa  <  1:329144/80‑1 (MQ=255)                                                                            |                                                                      GGTCATTATATTAACGTATTTTATAGCACTGCCGATATCACGCTCAAAAAACAACCACTGCTATTTTTAGCAAAGCTTATGGTATACTCCGCCGCCTTAACATTTTTCACCGCAAATTTTCATTGCAACATGACGAGGAAAATAA  >  NC_000913/1995673‑1995817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑