Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,409	A→G	E177G (GAA→GGA)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,409	0	A	G	100.0%	79.9 / NA	23	E177G (GAA→GGA)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

CGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015333‑2015484                                                                             |                                                                            cGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTa                                                                         >  2:332023/1‑81 (MQ=255)        gcCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATg                                                                  >  2:332026/1‑81 (MQ=255)            cgacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCtt                                                              <  1:331989/81‑1 (MQ=255)             gacgTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTg                                                             >  2:332029/1‑81 (MQ=255)                gTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTc                                                          >  2:332032/1‑81 (MQ=255)                gTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTc                                                          >  2:332022/1‑81 (MQ=255)                 tGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCg                                                         <  2:331990/81‑1 (MQ=255)                   aTGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGAc                                                       <  1:331991/81‑1 (MQ=255)                      ttGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGt                                                    <  1:331992/81‑1 (MQ=255)                        gCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTc                                                   <  2:331993/80‑1 (MQ=255)                        gCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTc                                                   <  2:331994/80‑1 (MQ=255)                              cGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAAtct                                            >  2:332028/1‑81 (MQ=255)                                          cggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCgcagca                                 >  1:332034/1‑80 (MQ=255)                                           ggcgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCaa                                >  2:332037/1‑80 (MQ=255)                                             cgTGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAAt                             >  2:332043/1‑81 (MQ=255)                                               tGCAGCCAGCCGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATgg                           <  2:331995/81‑1 (MQ=255)                                                   gccagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcgg                       <  2:331996/81‑1 (MQ=255)                                                   gccagccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGggcgg                       <  1:331997/81‑1 (MQ=255)                                                      agccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTTAGAATCTCAAGCGCAGCAAAATGGGCGGCGt                    >  2:332027/1‑81 (MQ=255)                                                      agccGCGCTGGCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGt                    <  2:331998/81‑1 (MQ=255)                                                               ggCGGAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACgg            >  1:332030/1‑80 (MQ=255)                                                                  ggAGCTGACCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGc        <  2:331999/81‑1 (MQ=255)                                                                         aCCGGAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAt  <  1:332000/80‑1 (MQ=255)                                                                             |                                                                            CGTCTGCGCCACGACGTGATGTTGCGTATCGCCACCTTTGGCGGCGTGCAGCCAGCCGCGCTGGCGGAGCTGACCGAAGTACTGAATGGCTTGCTCGACGGTCAGAATCTCAAGCGCAGCAAAATGGGCGGCGTGAGAACGGCAGCCGAAAT  >  NC_000913/2015333‑2015484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑