Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,048,809	(T)5→4	coding (524/645 nt)	gfcB ←	lipoprotein GfcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,048,805	0	T	.	100.0%	92.1 / NA	22	coding (528/645 nt)	gfcB	lipoprotein GfcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/12);  total (10/12)

AACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCT  >  NC_000913/1048727‑1048881                                                                               |                                                                             aaCCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttt                                                                            >  2:213153/1‑81 (MQ=255) aaCCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttt                                                                            >  2:213152/1‑81 (MQ=255)  aCCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttt                                                                            <  1:213124/80‑1 (MQ=255)   ccccTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttttc                                                                          >  1:213181/1‑80 (MQ=255)      cTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttttctgg                                                                       >  2:213162/1‑77 (MQ=255)      cTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGttttctgg                                                                       >  2:213159/1‑77 (MQ=255)         cgcCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCcg                                                                   >  1:213160/1‑81 (MQ=255)          gcCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCTAGAATGTGTTTTGCCACTG‑TTTTCTGGCcgc                                                                  >  2:213158/1‑81 (MQ=255)            cTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGt                                                                <  2:213125/81‑1 (MQ=255)             tAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTg                                                               >  2:213157/1‑81 (MQ=255)               aCATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTgg                                                              <  1:213127/80‑1 (MQ=255)               aCATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTgg                                                              <  2:213126/80‑1 (MQ=255)                 aTTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGACGCGTGGAAg                                                           <  2:213128/81‑1 (MQ=255)                    tGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCa                                                        >  2:213156/1‑81 (MQ=255)                        gACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACctc                                                    >  2:213166/1‑81 (MQ=255)                             tGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTc                                                 <  1:213129/79‑1 (MQ=255)                              gACGAACCTGGCCAGTAGCCGAATCGACCCGGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCt                                               <  2:213130/80‑1 (MQ=255)                              gACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCt                                               <  2:213131/80‑1 (MQ=255)                              gACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCt                                               <  1:213132/80‑1 (MQ=255)                              gACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTg                                              >  2:213164/1‑81 (MQ=255)                                cGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGc                                             >  1:213161/1‑80 (MQ=255)                                     cTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACa                                       <  1:213133/81‑1 (MQ=255)                                        gCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACAtt                                     <  1:213134/80‑1 (MQ=255)                                             tAGCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAg                               >  1:213174/1‑81 (MQ=255)                                               gCCGAATCGACCCAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGc                              <  2:213135/80‑1 (MQ=255)                                                         cccAGAATGTGTTTTGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGAcc                   <  1:213136/81‑1 (MQ=255)                                                                      ttGCCACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGc      >  2:213165/1‑81 (MQ=255)                                                                          cACTG‑TTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGtct  >  2:213163/1‑81 (MQ=255)                                                                               |                                                                             AACCCCTGCGCCTAACATTTGCCGACTTTGACGAACCTGGCCAGTAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCT  >  NC_000913/1048727‑1048881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑