Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,184,877	(T)5→4	coding (718/1137 nt)	potA ←	spermidine preferential ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,184,873	0	T	.	100.0%	99.7 / NA	24	coding (722/1137 nt)	potA	spermidine preferential ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/14);  total (10/14)

GCTGCTCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCC  >  NC_000913/1184799‑1184945                                                                           |                                                                         gctgctCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGGttttcgg                                                                    >  2:235421/1‑78 (MQ=255) gctgctCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGGttttcgg                                                                    >  1:235431/1‑78 (MQ=255)     ctCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGctct                                                               <  2:235390/81‑1 (MQ=255)     ctCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGctc                                                                <  1:235388/80‑1 (MQ=255)     atCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGctc                                                                <  2:235389/79‑1 (MQ=255)      tCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCtt                                                              >  1:235432/1‑81 (MQ=255)          cTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCg                                                            >  2:235438/1‑79 (MQ=255)          cTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCg                                                            >  2:235424/1‑79 (MQ=255)           tAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCg                                                            >  2:235449/1‑78 (MQ=255)             gACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTa                                                          <  1:235391/78‑1 (MQ=255)                gTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTc                                                    <  1:235392/81‑1 (MQ=255)                          aGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTg                                          >  1:235430/1‑81 (MQ=255)                                gTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCt                                    <  2:235393/81‑1 (MQ=255)                                       atatTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCaa                             <  2:235395/81‑1 (MQ=255)                                       atatTGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCaa                             <  2:235394/81‑1 (MQ=255)                                          ttGATTTCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGc                          <  2:235396/81‑1 (MQ=255)                                              tttCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGAcc                      <  1:235397/81‑1 (MQ=255)                                                tCGCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCAt                    >  2:235435/1‑81 (MQ=255)                                                  gCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATcg                  >  1:235433/1‑81 (MQ=255)                                                  gCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATc                   <  2:235399/80‑1 (MQ=255)                                                  gCCAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATc                   <  1:235398/80‑1 (MQ=255)                                                   ccAATGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATcgc                 <  1:235400/81‑1 (MQ=255)                                                     aaTGAAGCCGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATcgcg                >  2:235446/1‑80 (MQ=255)                                                            ccGGCAACAAACAGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATcacca        >  1:235436/1‑81 (MQ=255)                                                                 aacaaacaGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATc   >  2:235447/1‑81 (MQ=255)                                                                  acaaacaGG‑TTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATcc  <  1:235401/81‑1 (MQ=255)                                                                           |                                                                         GCTGCTCGTCTAGACGTTCGATGACAGTGGCGTTAAACATATTGATTTCGCCAATGAAGCCGGCAACAAACAGGTTTTTCGGCTCTTCGTAGATTTCACGCGGCGTGCCGTCTTGCTCAATGCGACCATCGCGCATCACCACAATCC  >  NC_000913/1184799‑1184945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑