Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,413,945	(A)6→5	intergenic (‑18/+39)	ralR ← / ← recT	Rac prophage; endodeoxyribonuclease toxin RalR/Rac prophage; recombinase, DNA renaturation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413,940	0	A	.	100.0%	42.5 / NA	11	intergenic (‑13/+44)	ralR/recT	Rac prophage; endodeoxyribonuclease toxin RalR/Rac prophage; recombinase, DNA renaturation
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/2);  total (9/2)

ACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACT  >  NC_000913/1413872‑1414010                                                                     |                                                                       aCCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTc                                                             >  1:268993/1‑79 (MQ=255)              gACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCAt                                              >  2:268992/1‑81 (MQ=255)              gACAACCACAAAATGGACATGGTTTAACATTGTCATATCACATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCAt                                              >  1:268991/1‑81 (MQ=255)                           tGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGc                                 >  1:268983/1‑81 (MQ=255)                                aTGGTTTAACATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAat                            >  2:269005/1‑81 (MQ=255)                                       aaCATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCct                     >  2:268997/1‑81 (MQ=255)                                        aCATTGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCctc                    >  1:268995/1‑81 (MQ=255)                                           ttGTCATATCTCATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGa                 <  1:268984/81‑1 (MQ=255)                                                   tctcATAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGa         >  1:269006/1‑81 (MQ=255)                                                       aTAATTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGAtt       <  1:268985/79‑1 (MQ=255)                                                          aTTTTTCTCAT‑AAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACt  >  2:268994/1‑81 (MQ=255)                                                                     |                                                                       ACCGTTACTGATGGACAACCACAAAATGGACATGGTTTAACATTGTCATATCTCATAATTTTTCTCATAAAAAATATTTCAAGTTGGCGGTGCATTACACCGCCAGGCTGAATTATTCCTCTGAATTATCGATTACACT  >  NC_000913/1413872‑1414010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑