Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,434,706	Δ1 bp	intergenic (‑300/+252)	ynaM ← / ← ttcC	Rac prophage; protein YnaM/putative tRNA 2‑thiocytidine (s(2)C32) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,434,706	0	G	.	100.0%	23.8 / NA	8	intergenic (‑300/+252)	ynaM/ttcC	Rac prophage; protein YnaM/putative tRNA 2‑thiocytidine (s(2)C32) synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (5/3);  total (5/3)

ATCTGAAGAAGAAAAATATCAAGGTGATATAGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGAGTGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTATGCAAGAACGTGCTGCGGTTGGTTTGACTTT  >  NC_000913/1434635‑1434777                                                                        |                                                                        aTCTGAAGAAGAAAAATATCAAGGTGATATAGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGA‑TGATGTATCt                                                               >  1:271656/1‑81 (MQ=16)      aagaagaaAAATATCAAGGTGATATAGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGA‑TGATGTATCTGaa                                                            >  1:271653/1‑79 (MQ=255)        gaagaaAAATATCAAGGTGATATAGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGA‑TGATGTATCTGAATAGt                                                        >  2:271654/1‑81 (MQ=9)                              taGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGA‑TGATGTATCTGAATAGTTGAAAAACCGCAGTCAcgtcgt                                  <  1:271644/81‑1 (MQ=255)                                gCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGA‑TGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTAt                                >  2:271664/1‑81 (MQ=255)                                                    gaggaATGAATGTGATGGGA‑TGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTATGCAAGAACGTGCTGCggttg            <  1:271645/81‑1 (MQ=255)                                                              tgtgATGGGA‑TGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTATGCAAGAACGTGCTGCGGTTGGTTTGACttt  <  2:271646/81‑1 (MQ=255)                                                               gtgATGGGA‑TGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTATGCAAGAACGTGCTGCGGTTGGTTTGACttt  >  2:271659/1‑80 (MQ=255)                                                                        |                                                                        ATCTGAAGAAGAAAAATATCAAGGTGATATAGCCTATATGCCTTTGATGCGGAGGAATGAATGTGATGGGAGTGATGTATCTGAATAGTTGAAAAACCGCAGTCACGTCGTATGCAAGAACGTGCTGCGGTTGGTTTGACTTT  >  NC_000913/1434635‑1434777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑