Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,487,583	(A)6→5	coding (349/801 nt)	ydcF →	DUF218 domain‑containing protein YdcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,487,578	0	A	.	100.0%	78.5 / NA	19	coding (344/801 nt)	ydcF	DUF218 domain‑containing protein YdcF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/9);  total (10/9)

ACTATCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGAAAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATT  >  NC_000913/1487502‑1487644                                                                             |                                                                   acTATCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGaaaaa                                                                <  2:279350/81‑1 (MQ=255)  cTATCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGaaaaat                                                               >  2:279397/1‑80 (MQ=255)     tCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGaaaaatct                                                             <  1:279353/80‑4 (MQ=255)     tCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGaaaaatc                                                              <  1:279351/79‑3 (MQ=255)     tCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGaaaaatc                                                              <  2:279352/79‑3 (MQ=255)                 gcagagcagaAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGAcc                                                <  1:279354/80‑1 (MQ=255)                 gcagagcagaAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGAc                                                 >  1:279396/1‑79 (MQ=255)                     agcagaAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTc                                            >  2:279389/1‑80 (MQ=255)                        agaAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTcaac                                         >  1:279380/1‑80 (MQ=255)                            gCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACt                                    <  1:279355/81‑1 (MQ=255)                            gCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACt                                    >  1:279403/1‑81 (MQ=255)                             cGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTg                                   <  1:700225/81‑1 (MQ=255)                              gACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGc                                  <  2:279356/81‑1 (MQ=255)                                 cATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGt                               >  1:279392/1‑81 (MQ=255)                                       ggCGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAac                         >  1:279393/1‑81 (MQ=255)                                         cGGATATCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAacg                        >  1:279390/1‑80 (MQ=255)                                               tCGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCtt                 <  1:279357/81‑1 (MQ=255)                                                cGCTCATCAGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCtt                 >  1:279401/1‑80 (MQ=255)                                                        aGTTCTGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATcgc        >  1:279399/1‑81 (MQ=255)                                                             tGGCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTAt   >  1:279395/1‑81 (MQ=255)                                                               gCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTa    <  1:279358/78‑1 (MQ=255)                                                               gCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTAtt  <  1:279359/80‑1 (MQ=255)                                                               gCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTAt   <  1:279361/79‑1 (MQ=255)                                                               gCACATTCCGCATG‑AAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTAt   <  1:279360/79‑1 (MQ=255)                                                                             |                                                                   ACTATCCGCACCACTGGCAGAGCAGAAGCGACCATCCTGGCGGATATCGCTCATCAGTTCTGGCACATTCCGCATGAAAAAATCTGGATTGAAGACCAGTCAACAAACTGCGGTGAAAACGCACGCTTTAGCATCGCGCTATT  >  NC_000913/1487502‑1487644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑