Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,489,696	(T)6→5	intergenic (+25/+17)	aldA → / ← gapC	aldehyde dehydrogenase A/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,489,691	0	T	.	100.0%	96.3 / NA	23	intergenic (+20/+22)	aldA/gapC	aldehyde dehydrogenase A/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (7/16);  total (7/16)

ACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATAT  >  NC_000913/1489620‑1489767                                                                        |                                                                             aCATGGCTTGCATGAATATCTGCAGACCCCGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTtcctcc                                                                    <  2:279640/81‑1 (MQ=255)  cATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCg                                                                   <  1:279641/81‑1 (MQ=255)      gCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCtg                                                               <  1:279642/81‑1 (MQ=255)          gCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTgc                                                            <  2:279643/80‑1 (MQ=255)                     tGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGtt                                                 >  2:279678/1‑80 (MQ=255)                               ggtggtTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAAtttt                                      <  1:279644/81‑1 (MQ=255)                                  ggtTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTc                                    >  1:279670/1‑80 (MQ=255)                                   gtTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCg                                   <  1:279645/80‑1 (MQ=255)                                    tttatttaCAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAg                                 <  1:279646/81‑1 (MQ=255)                                      tatttaCAGTCTTAATGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAgg                                <  1:279647/80‑1 (MQ=255)                                                    aTGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCtgt                 <  1:279648/81‑1 (MQ=255)                                                    aTGAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCtgt                 <  1:279649/81‑1 (MQ=255)                                                      gAGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCtgtgt               >  1:279671/1‑81 (MQ=255)                                                       aGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCtgtgtg              <  1:279650/81‑1 (MQ=255)                                                       aGTGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCtgtgt               <  2:279651/80‑1 (MQ=255)                                                         tGAAAGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGAc            <  1:279652/81‑1 (MQ=255)                                                           aaaGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACg           >  2:279675/1‑80 (MQ=255)                                                           aaaGAGGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGa          <  2:279653/81‑1 (MQ=255)                                                             agagGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAg        >  2:279683/1‑81 (MQ=255)                                                              gagGCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGc       >  2:279673/1‑81 (MQ=255)                                                                 gCGGAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCat    <  2:279654/81‑1 (MQ=255)                                                                   ggAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCatat  >  1:279672/1‑81 (MQ=255)                                                                   ggAGG‑TTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCatat  <  2:279655/81‑1 (MQ=255)                                                                        |                                                                             ACATGGCTTGCATGAATATCTGCAGACCCAGGTGGTTTATTTACAGTCTTAATGAGTGAAAGAGGCGGAGGTTTTTTCCTCCGCCTGTGCGCGTCAGAGTTTAGCGAATTTTTCGAGGGTGCGAATAAGCTGTGTGACGAAGCCATAT  >  NC_000913/1489620‑1489767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑