Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,490,954	(T)5→4	coding (53/531 nt)	cybB →	cytochrome b561

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,490,950	0	T	.	100.0%	60.3 / NA	15	coding (49/531 nt)	cybB	cytochrome b561
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (9/6);  total (9/6)

ATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTC  >  NC_000913/1490879‑1491021                                                                        |                                                                        aTTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGt                                                               <  1:279816/81‑1 (MQ=255)     tGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATc                                                           >  2:279831/1‑81 (MQ=255)      gTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCg                                                          >  2:279829/1‑81 (MQ=255)       ttAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGc                                                         <  2:279817/81‑1 (MQ=255)                   aTGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTgcg                                              >  1:279835/1‑80 (MQ=255)                      ttATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAAt                                          <  1:279818/81‑1 (MQ=255)                           gAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGt                                     >  2:279840/1‑81 (MQ=255)                              aataaataTTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTc                                  >  1:279833/1‑81 (MQ=255)                                taaataTTCAAGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGt                                <  2:279819/81‑1 (MQ=255)                                         aaGGTTACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTttcttc                       >  1:279838/1‑81 (MQ=255)                                             ttACAAATCAGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCAc                   >  2:279837/1‑81 (MQ=255)                                                      aGCATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGAt           <  1:759467/80‑1 (MQ=255)                                                        cATTCACTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCgg        >  2:279841/1‑81 (MQ=255)                                                             aCTGGCTGGTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACt   >  1:279839/1‑81 (MQ=255)                                                              ctggctggTC‑TTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACtc  <  2:279820/81‑1 (MQ=255)                                                                        |                                                                        ATTATGTTAAAGCAGGAAATGTTATGGAAAATAAATATTCAAGGTTACAAATCAGCATTCACTGGCTGGTCTTTTTACTGGTTATCGCAGCGTATTGCGCAATGGAGTTTCGTGGTTTCTTCCCACGTAGCGATCGGCCACTC  >  NC_000913/1490879‑1491021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑