Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,577,751	(T)5→4	coding (1592/1686 nt)	yddA ←	ABC transporter family protein YddA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,577,747	0	T	.	100.0%	33.1 / NA	9	coding (1596/1686 nt)	yddA	ABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/3);  total (6/3)

AATGTCACAAATATCATCGGCCAGGTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAG  >  NC_000913/1577672‑1577815                                                                            |                                                                     aaTGTCACAAATATCATCGGCCAGGTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAGttttca                                                                 >  2:291487/1‑79 (MQ=255)                atcGGCCAGGTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGt                                                 <  2:291463/81‑1 (MQ=255)                    gCCAGGTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGc                                             >  1:291479/1‑81 (MQ=255)                         gTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATa                                        <  2:291464/81‑1 (MQ=255)                          ttCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAg                                       >  2:291484/1‑81 (MQ=255)                               gACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAGCctct                                  >  1:291493/1‑81 (MQ=255)                                          gATGTGTAACCATAATGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTc                          >  2:291495/1‑78 (MQ=255)                                                         tGACGCCGCTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTc        <  1:291465/81‑1 (MQ=255)                                                            cgccgcTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGt      >  2:291490/1‑80 (MQ=255)                                                                gcTTGTGGGTAG‑TTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAg  >  1:291486/1‑80 (MQ=255)                                                                            |                                                                     AATGTCACAAATATCATCGGCCAGGTTCCAGACACCGGGTTGATGTGTAACCATAATGACGCCGCTTGTGGGTAGTTTTTCACGCACTAAACGCAGTAAGCGGATAGCCTCTTGTTCCTCAAGATGAGAGGTAGTTTCGTCAAG  >  NC_000913/1577672‑1577815

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑