Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	42,759	Δ1 bp	coding (357/771 nt)	fixA →	putative electron transfer flavoprotein FixA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	42,759	0	C	.	100.0%	159.6 / NA	37	coding (357/771 nt)	fixA	putative electron transfer flavoprotein FixA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/22);  total (15/22)

CCGCAACAAACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGGCGATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGCAGTTAA  >  NC_000913/42691‑42833                                                                     |                                                                           ccGCAACAAACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGttcttc                                                                 <  1:9652/79‑1 (MQ=255) ccGCAACAAACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGttcttc                                                                 <  2:9653/79‑1 (MQ=255)   gCAACAAACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGAc                                                             >  2:9726/1‑81 (MQ=255)       caaACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCtt                                                          >  1:9718/1‑80 (MQ=255)         aaCGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTAt                                                       <  1:9654/81‑1 (MQ=255)         aaCGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTAt                                                       >  1:9709/1‑81 (MQ=255)           cGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGc                                                     >  1:9711/1‑81 (MQ=255)           cGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGc                                                     >  1:9717/1‑81 (MQ=255)            ggCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGcc                                                    >  2:9715/1‑81 (MQ=255)                     aCTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGtt                                           <  1:9655/81‑1 (MQ=255)                          cTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTct                                      <  1:9656/81‑1 (MQ=255)                             cAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTctgct                                   >  1:9723/1‑81 (MQ=255)                             cAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTctg                                     <  2:9657/79‑1 (MQ=255)                              aGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTctgctg                                  >  1:9724/1‑81 (MQ=255)                                  gccCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCtggtgg                              <  1:9659/81‑1 (MQ=255)                                  gccCAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCtggtgg                              <  1:9658/81‑1 (MQ=255)                                   cccAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTggg                             <  2:9660/81‑1 (MQ=255)                                     cAGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCg                           <  1:9661/81‑1 (MQ=255)                                      aGAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGa                          <  2:9662/81‑1 (MQ=255)                                       gAAAGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGaa                         <  2:9663/81‑1 (MQ=255)                                        aaaGCAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGaaa                        >  2:9716/1‑81 (MQ=255)                                            cAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCt                    <  2:9664/81‑1 (MQ=255)                                            cAGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCt                    <  2:9665/81‑1 (MQ=255)                                             aGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCt                    >  1:9725/1‑80 (MQ=255)                                             aGGCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCt                    >  2:9719/1‑80 (MQ=255)                                               gCTTTGATCTGATCCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCaa                 <  2:9666/81‑1 (MQ=255)                                                   tgatctgatcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATAtt             >  1:9720/1‑81 (MQ=255)                                                    gatctgatcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATAtt             <  2:9668/80‑1 (MQ=255)                                                    gatctgatcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATAtt             <  2:9667/80‑1 (MQ=255)                                                     atctgatcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTc            <  1:9669/80‑1 (MQ=255)                                                      tctgatcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCg          >  1:9721/1‑81 (MQ=255)                                                          atcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTcc           <  1:9670/76‑1 (MQ=255)                                                          atcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGc        <  1:9672/79‑1 (MQ=255)                                                          atcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGc        <  1:9671/79‑1 (MQ=255)                                                          atcCTCTGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGCAg      >  1:9727/1‑81 (MQ=255)                                                             ctctGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGCAGtt    <  1:9673/80‑1 (MQ=255)                                                              tctGTGG‑GATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGCAGTTaa  >  2:9722/1‑81 (MQ=255)                                                                     |                                                                           CCGCAACAAACGGCGAGCGCACTGGCTGCAGCCGCCCAGAAAGCAGGCTTTGATCTGATCCTCTGTGGCGATGGTTCTTCCGACCTTTATGCCCAGCAGGTTGGTCTGCTGGTGGGCGAAATCCTCAATATTCCGGCAGTTAA  >  NC_000913/42691‑42833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑