Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,915,177	(T)6→5	coding (358/699 nt)	proQ ←	RNA chaperone ProQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,915,172	0	T	.	100.0%	137.9 / NA	32	coding (363/699 nt)	proQ	RNA chaperone ProQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (19/13);  total (19/13)

CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGC  >  NC_000913/1915092‑1915244                                                                                 |                                                                         cggcgTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGt                                                                          >  2:337345/1‑81 (MQ=255)  ggcgtggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTTCGGCAGCTTCGCGtt                                                                         >  2:337351/1‑81 (MQ=255)      tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                     <  1:337291/81‑2 (MQ=255)      tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                     <  1:337290/81‑2 (MQ=255)      tggcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttc                                                                     <  2:337289/81‑2 (MQ=255)       ggcgtAGTCGGACGTGGCTTGCGTTAGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttcg                                                                    >  2:337326/1‑79 (MQ=255)        gcgtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGtttttcg                                                                    <  1:337292/80‑3 (MQ=255)          gtAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCtt                                                                <  2:337293/81‑1 (MQ=255)              tCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTg                                                            <  1:337294/81‑1 (MQ=255)               cGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGt                                                           >  1:337335/1‑81 (MQ=255)                 gACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTc                                                         <  1:337295/81‑1 (MQ=255)                    gTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAg                                                       >  1:337347/1‑80 (MQ=255)                    gTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAg                                                       >  2:337348/1‑80 (MQ=255)                    gTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGc                                                      <  1:337296/81‑1 (MQ=255)                       gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACg                                                   >  2:337344/1‑81 (MQ=255)                       gCTTGCGTTCGCGGCGCGGTGCGTCTTCTTCCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCAc                                                    <  1:337297/80‑1 (MQ=255)                            cGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCtgt                                               <  1:337298/80‑1 (MQ=255)                              ttCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGcc                                            >  2:337340/1‑81 (MQ=255)                                   ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                        >  2:337342/1‑80 (MQ=255)                                   ggcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGaa                                        >  1:337353/1‑80 (MQ=255)                                    gcgcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAaca                                      >  1:337337/1‑81 (MQ=255)                                      gcggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcg                                    <  2:337299/81‑1 (MQ=255)                                        ggTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACAcgcg                                  <  1:337300/81‑1 (MQ=255)                                           gCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt                               >  2:337343/1‑81 (MQ=255)                                            cGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCtt                               <  2:337301/80‑1 (MQ=255)                                              tcttctTTCTCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCg                            <  2:337302/81‑1 (MQ=255)                                                  ctttctCACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGcttc                        >  2:337346/1‑81 (MQ=255)                                                        cACCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAg                  >  2:337341/1‑81 (MQ=255)                                                         aCCAGCAGTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAg                  <  1:337303/80‑1 (MQ=255)                                                             gcagTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAgctgct             <  1:337304/81‑1 (MQ=255)                                                               agTTGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTg           <  2:337305/81‑1 (MQ=255)                                                                 ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc          >  1:337358/1‑80 (MQ=255)                                                                 ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc          >  1:337350/1‑80 (MQ=255)                                                                 ttGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc          >  1:337339/1‑80 (MQ=255)                                                                  tGCGGCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGc          >  1:337360/1‑79 (MQ=255)                                                                     ggCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCa     >  1:337367/1‑81 (MQ=255)                                                                     ggCAGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCa     >  2:373975/1‑81 (MQ=255)                                                                        aGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGc  >  1:337356/1‑81 (MQ=255)                                                                        aGCTTCGCG‑TTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGc  >  1:337352/1‑81 (MQ=255)                                                                                 |                                                                         CGGCGTGGCGTAGTCGGACGTGGCTTGCGTTCGCGGCGCGGTGCGTCTTCTTTCTCACCAGCAGTTGCGGCAGCTTCGCGTTTTTTCGCTTGCTGTTCAGCACGCTGTGCCTGAACACGCGCTTTCGCTTCTTCAAGCTGCTTGCGAGCATGC  >  NC_000913/1915092‑1915244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑