Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,044,771	Δ1 bp	intergenic (+147/‑167)	asnT → / → yeeJ	tRNA‑Asn/inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,044,768	0	C	.	100.0%	110.7 / NA	26	intergenic (+144/‑170)	asnT/yeeJ	tRNA‑Asn/inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (15/11);  total (15/11)

GGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGT  >  NC_000913/2044696‑2044844                                                                         |                                                                             ggTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGAt                                                                      >  2:357945/1‑80 (MQ=255)    ttttAACATCTTAAGCGCTCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCTATCACCATTTT‑CCCTCGATtat                                                                   >  2:357947/1‑80 (MQ=255)     tttAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATtata                                                                  <  2:357919/80‑1 (MQ=255)                aaGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGtt                                                       <  1:357920/80‑1 (MQ=255)                 aGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTAt                                                     >  1:357961/1‑81 (MQ=255)                 aGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTAt                                                     >  1:357955/1‑81 (MQ=255)                   cgcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTAtt                                                    >  1:357953/1‑80 (MQ=255)                   cgcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTc                                                   <  1:357921/81‑1 (MQ=255)                    gcCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTAtt                                                    >  1:357952/1‑79 (MQ=255)                          gACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGtc                                            >  1:357957/1‑81 (MQ=255)                           aCCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGtct                                           >  1:357950/1‑81 (MQ=255)                             cTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTcc                                         <  1:357922/81‑1 (MQ=255)                              tttATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                        >  1:357951/1‑81 (MQ=255)                                tATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                        <  2:357923/79‑1 (MQ=255)                                tATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTccc                                        <  1:357924/79‑1 (MQ=255)                                  tGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCctct                                    <  2:357925/81‑1 (MQ=255)                                       gAGGGCGTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCa                                <  1:357926/80‑1 (MQ=255)                                             gTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcaca                         >  2:357965/1‑81 (MQ=255)                                             gTTTTGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTcaca                         >  1:357975/1‑81 (MQ=255)                                                 tGCTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACAccc                      >  1:357956/1‑80 (MQ=255)                                                   cTATGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCaaa                   <  1:357927/81‑1 (MQ=255)                                                      tGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACt                <  2:357929/81‑1 (MQ=255)                                                      tGAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACt                <  1:357928/81‑1 (MQ=255)                                                       gAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTg               >  1:357963/1‑81 (MQ=255)                                                       gAACGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTg               >  1:357962/1‑81 (MQ=255)                                                          cGCCATCACCATTTT‑CCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGcc             >  1:357964/1‑80 (MQ=255)                                                                     attttCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGt  <  1:357930/80‑1 (MQ=255)                                                                         |                                                                             GGTTTTTAACATCTTAAGCGCCCTCGACCTTTATGGTTGAGGGCGTTTTGCTATGAACGCCATCACCATTTTCCCCTCGATTATAAAACTTGAGTTATTCAGTAGTCTCCCCTCTTGCAACTCACACCCAAAACTGCCTAACGAAAAGT  >  NC_000913/2044696‑2044844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑