Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,632	Δ1 bp	coding (354/1947 nt)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,629	0	A	.	100.0%	107.6 / NA	25	coding (357/1947 nt)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/16);  total (9/16)

GTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTACCA  >  NC_000913/2150557‑2150704                                                                         |                                                                            gTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCAc                                                                    >  1:375400/1‑81 (MQ=255)    aCCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGc                                                                  <  2:375358/80‑1 (MQ=255)        acgacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAAt                                                             >  2:375387/1‑81 (MQ=255)        acgacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAAt                                                             <  2:375359/81‑1 (MQ=255)          gacgTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAAt                                                             >  2:375398/1‑79 (MQ=255)              tGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGt                                                       <  2:375361/81‑1 (MQ=255)              tGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGt                                                       <  1:375360/81‑1 (MQ=255)                  cGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAAct                                                   >  2:375390/1‑81 (MQ=255)                    tGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAActct                                                 <  2:375362/81‑1 (MQ=255)                         gTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGcg                                             <  2:375363/80‑1 (MQ=255)                          tGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGcgac                                           <  1:375364/81‑1 (MQ=255)                          tGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGcgac                                           <  1:375365/81‑1 (MQ=255)                               cGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGAtgt                                      <  2:375366/81‑1 (MQ=255)                                gTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGAtgtg                                     >  1:375397/1‑81 (MQ=255)                                  aGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGcc                                   >  1:375403/1‑81 (MQ=255)                                   gCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGcc                                   <  1:375367/80‑1 (MQ=255)                                     aaaaGATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCggg                                >  2:375395/1‑81 (MQ=255)                                         gATGAAGCAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCtat                            <  1:375368/81‑1 (MQ=255)                                                cAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCat                     <  1:375369/81‑1 (MQ=255)                                                cAATATTGCGCGCAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCa                      >  2:375405/1‑80 (MQ=255)                                                        cgcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATc              <  1:375370/80‑1 (MQ=255)                                                         gcgcAACATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCgg            <  1:375371/81‑1 (MQ=255)                                                             aaCATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCt        <  1:375372/81‑1 (MQ=255)                                                             aaCATAGAGTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCt        >  2:375401/1‑81 (MQ=255)                                                                   gagTAG‑AAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTACCa  <  1:375373/81‑1 (MQ=255)                                                                         |                                                                            GTCACCCACGACGTGCCCGTGCTCGTGAACCGTAGCAAAAGATGAAGCAATATTGCGCGCAACATAGAGTAGAAAATCCCACGCACAATGAGGGTAACTCTGGCGACGATGTGCCGGGCTATAGATCATATGAATCGGTTCTTTACCA  >  NC_000913/2150557‑2150704

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑