Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,197,358	(A)5→4	intergenic (+60/‑2921)	yehF → / → yehI	putative protein YehF/DUF4132 domain‑containing protein YehI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,197,354	0	A	.	100.0%	95.4 / NA	23	intergenic (+56/‑2925)	yehF/yehI	putative protein YehF/DUF4132 domain‑containing protein YehI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (9/14);  total (9/14)

GAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCG  >  NC_000913/2197275‑2197422                                                                                |                                                                     gAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGcaca                                                                      >  1:383518/1‑80 (MQ=255)    cAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACaaaac                                                                  >  2:383521/1‑80 (MQ=255)         ttCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTAt                                                             <  2:383496/80‑1 (MQ=255)                   gAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTc                                                  >  1:383516/1‑81 (MQ=255)                    aaTGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTc                                                  <  1:383497/80‑1 (MQ=255)                      tGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCcac                                               <  1:383498/81‑1 (MQ=255)                       gACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCcaca                                              >  1:383524/1‑81 (MQ=255)                               cGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTc                                         >  2:383527/1‑78 (MQ=255)                               cGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTc                                         >  1:383522/1‑78 (MQ=255)                                   aaCATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAAt                                   <  2:383499/80‑1 (MQ=255)                                     cATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATggc                                <  1:383500/81‑1 (MQ=255)                                         tATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATggcggc                             <  2:383501/80‑1 (MQ=255)                                         tATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATggcggc                             <  2:383502/80‑1 (MQ=255)                                          aTCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCaa                           >  1:383528/1‑81 (MQ=255)                                           tCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCaa                           <  2:383503/80‑1 (MQ=255)                                              cTGACGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCAt                       <  2:383504/81‑1 (MQ=255)                                                 aCGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCAtt                      <  2:383505/79‑1 (MQ=255)                                                 aCGGAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTaa                    >  1:383517/1‑81 (MQ=255)                                                   ggAAGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAAtt                  >  2:383519/1‑81 (MQ=255)                                                     aaGAGTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATGGc                >  2:383526/1‑81 (MQ=255)                                                        agTCTCTCTGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCttt             <  2:383506/81‑1 (MQ=255)                                                            tctctGGCAAAAATGTGCAC‑AAAGCTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtt          <  1:383507/80‑1 (MQ=255)                                                              tctGGCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGtta         <  1:383508/79‑1 (MQ=255)                                                                  gCAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTAccc   >  2:383520/1‑81 (MQ=255)                                                                   cAAAAATGTGCAC‑AAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCg  <  2:383509/81‑1 (MQ=255)                                                                                |                                                                     GAGCAATATTCCAGATTTGAATGACGCCTACGTAAACATTTATCACTGACGGAAGAGTCTCTCTGGCAAAAATGTGCACAAAAACTTATTGCCGCAATTCCACATATTCCAGAATGGCGGCAACCATTAATTGCTTTGTTATTACCCG  >  NC_000913/2197275‑2197422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑