Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,411,993	Δ1 bp	coding (97/651 nt)	hxpA ←	hexitol phosphatase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,991	0	C	.	100.0%	109.5 / NA	26	coding (99/651 nt)	hxpA	hexitol phosphatase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/13);  total (13/13)

GATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCA  >  NC_000913/2411911‑2412066                                                                                 |                                                                            gATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAc                                                                             >  2:423988/1‑81 (MQ=255)  aTTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAc                                                                             >  2:423985/1‑80 (MQ=255)     tGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAAccat                                                                          >  1:423994/1‑78 (MQ=255)        ccGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACg                                                                     <  2:423946/81‑1 (MQ=255)                   tGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGt                                                          >  2:423991/1‑81 (MQ=255)                      cgcAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGc                                                       <  1:423947/81‑1 (MQ=255)                      cgcAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGc                                                       <  2:423948/81‑1 (MQ=255)                           agagGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCAc                                                  <  2:423949/81‑1 (MQ=255)                            gagGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACg                                                 >  1:424003/1‑81 (MQ=255)                               gTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGccc                                              <  1:423950/81‑1 (MQ=255)                                   tCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTc                                          >  1:423986/1‑81 (MQ=255)                                    cGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTc                                          >  1:423993/1‑80 (MQ=255)                                     gCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTa                                        >  2:424002/1‑81 (MQ=255)                                      ccTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTAc                                       >  2:423992/1‑81 (MQ=255)                                                gTGAATGAAAGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGcaggcagg                             >  2:423977/1‑81 (MQ=255)                                                       aaaGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATcc                       <  1:423951/80‑1 (MQ=255)                                                         aGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccacc                    <  2:423952/81‑1 (MQ=255)                                                         aGCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccac                     >  2:424007/1‑80 (MQ=255)                                                          gCCAGCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATccac                     >  1:423999/1‑79 (MQ=255)                                                              gCACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGt               <  2:423953/81‑1 (MQ=255)                                                               cACCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGtt              <  1:423954/81‑1 (MQ=255)                                                                aCCTCTTCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTc             >  1:424010/1‑81 (MQ=255)                                                                     ttCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCAt          <  2:423955/79‑1 (MQ=255)                                                                     ttCCGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATc         >  2:424006/1‑80 (MQ=255)                                                                       ccGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCa        <  2:423956/79‑1 (MQ=255)                                                                        cGGCGCTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAg      <  1:423957/80‑1 (MQ=255)                                                                          gcgcTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATc   <  2:423958/81‑1 (MQ=255)                                                                            gcTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCa  >  2:424001/1‑80 (MQ=255)                                                                            gcTAA‑CCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCa  >  1:423996/1‑80 (MQ=255)                                                                                 |                                                                            GATTTGCCCGCCATAAAATGGCGCAGAGAGGTGATCGCCTGTTTACCGTGAATGAAAGCCAGCACCTCTTCCGGCGCTAACCCATGACGTCTGGCCCAGTTGCTCCACGCCCGTTCTACCGCAGGCAGGGAATCCACCAGCGTTCCATCAAGATCA  >  NC_000913/2411911‑2412066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑