Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,367	(A)5→4	coding (466/2190 nt)	pdeA ←	putative c‑di‑GMP phosphodiesterase PdeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,363	0	A	.	100.0%	65.2 / NA	16	coding (470/2190 nt)	pdeA	putative c‑di‑GMP phosphodiesterase PdeA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (8/8);  total (8/8)

GTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATC  >  NC_000913/2517296‑2517434                                                                    |                                                                        gtAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATc                                                           >  2:443216/1‑81 (MQ=255)  tAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCt                                                          >  1:443229/1‑81 (MQ=255)   aaaTCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCtt                                                         >  2:443219/1‑81 (MQ=255)        aGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGc                                                     <  1:443190/80‑1 (MQ=255)        aGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGc                                                     <  2:443191/80‑1 (MQ=255)                      aaGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAaagaag                                      <  1:443192/81‑1 (MQ=255)                       aGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGc                                     >  2:443205/1‑81 (MQ=255)                             aGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTcccc                                <  1:443193/80‑1 (MQ=255)                                aaaTCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCcac                              >  2:443230/1‑79 (MQ=255)                                 aaTCAACGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCcac                              >  2:443227/1‑78 (MQ=255)                                      aCGACCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACa                      >  1:443235/1‑81 (MQ=255)                                         aCCGTGAAAATGGCATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACAtgc                   >  1:443220/1‑81 (MQ=255)                                                      cATCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGcc      <  1:443194/81‑1 (MQ=255)                                                       aTCCGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCg     <  2:443195/81‑1 (MQ=255)                                                          cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGAt   <  2:443197/80‑1 (MQ=255)                                                          cGCATCGCCG‑AAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATc  <  2:443196/81‑1 (MQ=255)                                                                    |                                                                        GTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCCACAAGATACATGCTGCATTTGATGCCGATC  >  NC_000913/2517296‑2517434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑