Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,569,793	Δ1 bp	coding (545/837 nt)	eutJ ←	putative chaperonin, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,569,791	0	A	.	100.0%	143.5 / NA	33	coding (547/837 nt)	eutJ	putative chaperonin, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (12/21);  total (12/21)

TTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTT  >  NC_000913/2569715‑2569866                                                                             |                                                                            ttttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGaata                                                                          <  2:453689/80‑3 (MQ=255)   ttCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACgg                                                                      >  1:453732/1‑81 (MQ=255)         cacCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGAttt                                                                <  1:453691/81‑1 (MQ=255)         cacCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGAttt                                                                >  1:453724/1‑81 (MQ=255)         cacCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGAtt                                                                 <  2:453690/80‑1 (MQ=255)           ccGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTcc                                                              >  1:453738/1‑81 (MQ=255)            cGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCg                                                             <  1:453692/81‑1 (MQ=255)                  cACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCgag                                                        <  1:453693/80‑1 (MQ=255)                   aCCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGagag                                                      <  2:453695/81‑1 (MQ=255)                   aCCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGagag                                                      <  1:453694/81‑1 (MQ=255)                     cGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTc                                                    <  1:453696/81‑1 (MQ=255)                       cAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCag                                                  <  1:453697/81‑1 (MQ=255)                         ggCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCagag                                                <  2:453698/81‑1 (MQ=255)                           ccAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCagagag                                              <  1:453699/81‑1 (MQ=255)                            cAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCaga                                                 >  2:453736/1‑77 (MQ=255)                                    ttCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAgatgtg                                         <  1:453700/77‑1 (MQ=255)                                      cACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTcc                                   >  2:453751/1‑81 (MQ=255)                                       aCCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTccg                                  >  2:453741/1‑81 (MQ=255)                                          gTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTccgccg                               <  1:453701/81‑1 (MQ=255)                                             aCCGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTg                            <  1:453702/81‑1 (MQ=255)                                              ccGCGCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTgg                           >  2:453743/1‑81 (MQ=255)                                               cgcgCTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGc                          >  1:453754/1‑81 (MQ=255)                                                gcgcTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCt                         <  1:453703/81‑1 (MQ=255)                                                  gcTTGTACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTc                       <  2:453704/81‑1 (MQ=255)                                                       tACTGCTCCGCCTCTTCCAGCG‑AATACGGCGTTTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccg                  >  1:453734/1‑81 (MQ=255)                                                       tACTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATcc                   <  2:453705/80‑1 (MQ=255)                                                         cTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccgcc                >  1:453745/1‑81 (MQ=255)                                                         cTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccgcc                >  1:453748/1‑81 (MQ=255)                                                         cTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccgcc                <  1:453707/81‑1 (MQ=255)                                                         cTGCTCCGCCTCTTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATccgcc                <  1:453706/81‑1 (MQ=255)                                                                  ctctTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGt        >  1:453744/1‑80 (MQ=255)                                                                  ctctTCCAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTc       <  1:453708/81‑1 (MQ=255)                                                                       ccAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCt   <  1:453709/80‑1 (MQ=255)                                                                        cAGCG‑AATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCtt  <  1:453710/80‑1 (MQ=255)                                                                             |                                                                            TTTTCGTACACCGGTTTCACCGCAGGCCAAATCTCTTCACCGTGACCGCGCTTGTACTGCTCCGCCTCTTCCAGCGAAATACGGCGATTTCCGGCGAGAGTCAGAGAGATGTGATGTCCGCCGGTGGCTTCATCCGCCGAGTACGTCACCTT  >  NC_000913/2569715‑2569866

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑