Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,659,399	Δ1 bp	coding (69/516 nt)	hscB ←	co‑chaperone for [Fe‑S] cluster biosynthesis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,659,396	0	A	.	100.0%	115.5 / NA	27	coding (72/516 nt)	hscB	co‑chaperone for [Fe‑S] cluster biosynthesis
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (13/14);  total (13/14)

CTGTACGGCGGCGAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACAT  >  NC_000913/2659321‑2659472                                                                            |                                                                             cTGTACGGCGGCGAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTGaaacg                                                                          >  2:473188/1‑78 (MQ=255)       ggcggcGAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAggtt                                                                   >  2:473180/1‑78 (MQ=255)       ggcggcGAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCt                                                                   >  1:473186/1‑80 (MQ=255)            cgAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAgcgc                                                             <  1:473139/81‑1 (MQ=255)             gAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGcc                                                            <  1:473140/81‑1 (MQ=255)             gAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGcc                                                            >  2:473176/1‑81 (MQ=255)                ttgttCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTgg                                                         <  2:473141/81‑1 (MQ=255)                    tCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTAt                                                     >  2:473190/1‑81 (MQ=255)                      cGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCg                                                   <  1:473142/81‑1 (MQ=255)                       gCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATc                                                    >  2:473199/1‑79 (MQ=255)                       gCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATc                                                    >  2:473194/1‑79 (MQ=255)                       gCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGa                                                  <  1:473143/81‑1 (MQ=255)                         cTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGt                                                <  1:473144/81‑1 (MQ=255)                                      gCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGggca                                    <  2:473145/80‑1 (MQ=255)                                      gCGAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGggca                                    <  1:473146/80‑1 (MQ=255)                                        gAATTTATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGggcagg                                  >  1:473198/1‑80 (MQ=255)                                            ttATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGc                             >  1:473172/1‑81 (MQ=255)                                             tATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGc                             <  1:473148/80‑1 (MQ=255)                                             tATCAGGATGATACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGc                             <  1:473147/80‑1 (MQ=255)                                                   gatgatACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAagag                      >  2:473197/1‑81 (MQ=255)                                                    atgatACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATTGAGTTGATAGCGGGCAGGCAAGCCAAAGAgg                     >  2:473206/1‑81 (MQ=255)                                                        tACTGACGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGa                 <  1:473149/81‑1 (MQ=255)                                                             aCGTTGTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTa             <  2:473150/80‑1 (MQ=255)                                                                  gTAGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCa        <  2:473151/80‑1 (MQ=255)                                                                    aGATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATaa     >  1:473193/1‑81 (MQ=255)                                                                     gATCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATaa     >  2:473192/1‑80 (MQ=255)                                                                      aTCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACa   >  2:473191/1‑81 (MQ=255)                                                                       tCCTG‑AAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACAt  <  2:473152/81‑1 (MQ=255)                                                                            |                                                                             CTGTACGGCGGCGAGTTGTTCCGCCTGGCTTCCGCTGGCGAATTTATCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACAT  >  NC_000913/2659321‑2659472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑