Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,789	Δ1 bp	intergenic (+80/‑6)	nadK → / → recN	NAD kinase/DNA repair protein RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,786	0	A	.	100.0%	105.4 / NA	25	intergenic (+77/‑9)	nadK/recN	NAD kinase/DNA repair protein RecN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/6);  total (19/6)

ATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAG  >  NC_000913/2751709‑2751859                                                                              |                                                                          aTTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGaaa                                                                         <  1:492975/80‑1 (MQ=255)  ttttACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGaaacg                                                                       <  2:492976/81‑3 (MQ=255)      aCGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTa                                                                  >  1:493002/1‑81 (MQ=255)        gCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATg                                                                >  2:493001/1‑81 (MQ=255)         ccAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGt                                                               >  2:492995/1‑81 (MQ=255)                ctTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGcac                                                         >  2:493009/1‑80 (MQ=255)                 tttACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACaa                                                       >  1:493008/1‑81 (MQ=255)                     cTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGa                                                   >  2:493003/1‑81 (MQ=255)                        tatataAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCa                                                >  1:492996/1‑81 (MQ=255)                          tataAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCAt                                               <  2:492977/80‑1 (MQ=255)                                aCCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAAc                                        >  1:492999/1‑81 (MQ=255)                                aCCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAAc                                        <  2:492978/81‑1 (MQ=255)                                 ccAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACt                                       <  1:492979/81‑1 (MQ=255)                                  cAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACtt                                      <  2:492980/81‑1 (MQ=255)                                      ttATACTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGc                                   >  2:493013/1‑80 (MQ=255)                                         taCTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTAt                                <  2:492981/80‑1 (MQ=255)                                          aCTGTACACAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTAtcg                              >  2:493004/1‑81 (MQ=255)                                               acacAATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCGTCAGCAACTTTGCTAtcgttcgt                         >  1:493014/1‑81 (MQ=255)                                                 acaATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTAtcgttcgt                         >  2:493015/1‑79 (MQ=255)                                                  caATAACAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAg                      >  1:493016/1‑81 (MQ=255)                                                      aaCAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCtt                   >  1:493010/1‑80 (MQ=255)                                                      aaCAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCtt                   >  2:493020/1‑80 (MQ=255)                                                       aCAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCtt                   >  2:493023/1‑79 (MQ=255)                                                       aCAGTAATGGTTTTTCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTga                 <  1:492982/81‑1 (MQ=255)                                                                   tttCATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCa     >  1:493012/1‑81 (MQ=255)                                                                      cATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAg  >  2:493006/1‑81 (MQ=255)                                                                      cATACAGG‑AAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAg  >  2:493018/1‑81 (MQ=255)                                                                              |                                                                          ATTTTACGCCAGCCTCTTTACTGTATATAAAACCAGTTTATACTGTACACAATAACAGTAATGGTTTTTCATACAGGAAAACGACTATGTTGGCACAACTGACCATCAGCAACTTTGCTATCGTTCGTGAGCTTGAGATTGATTTTCATAG  >  NC_000913/2751709‑2751859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑