Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,632	(A)7→6	coding (1315/1704 nt)	yfjW →	CP4‑57 prophage; uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,626	0	A	.	100.0%	28.9 / NA	8	coding (1309/1704 nt)	yfjW	CP4‑57 prophage; uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/4);  total (4/4)

CTTGTCCTAAAACCAGATGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTA  >  NC_000913/2774548‑2774682                                                                               |                                                         cTTGTCCTAAAACCAGATGATATTATCAATTACAACAATATCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATaa                                                         >  1:496969/1‑80 (MQ=255)       cTAAAACCAGATGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATaaaaaacat                                                  <  2:496954/81‑4 (MQ=255)         aaaaCCAGATGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATAc                                               >  1:496965/1‑81 (MQ=255)                       ttattaATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATa                                  <  2:496955/80‑1 (MQ=255)                                 caacaaTAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATatga                        <  1:496956/80‑1 (MQ=255)                                  aacaaTAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATatgatg                      <  2:496957/81‑1 (MQ=255)                                      aTAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATATGATGACa                   >  1:496968/1‑80 (MQ=255)                                      aTAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATATGATGACAt                  <  2:496958/81‑1 (MQ=255)                                        aGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATATGATGACAt                  >  2:496972/1‑79 (MQ=255)                                                      actCAAGACTGATTTTGCGTTCTAT‑AAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTa  >  2:496970/1‑81 (MQ=255)                                                                               |                                                         CTTGTCCTAAAACCAGATGATATTATTAATTACAACAATAGCCCTTCCGTACTACTCAAGACTGATTTTGCGTTCTATAAAAAAACATACCAGAAGCTTGATAAAATATATGATGACATAAGCAATGGAAAATTA  >  NC_000913/2774548‑2774682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑