Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,940,603	Δ1 bp	coding (641/1101 nt)	rlmM ←	23S rRNA 2'‑O‑ribose C2498 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,940,601	0	C	.	100.0%	142.9 / NA	33	coding (643/1101 nt)	rlmM	23S rRNA 2'‑O‑ribose C2498 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/19);  total (14/19)

GTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGT  >  NC_000913/2940521‑2940671                                                                                 |                                                                       gTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGc                                                                        <  2:532419/81‑1 (MQ=255)  ttGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcc                                                                       <  2:532420/81‑1 (MQ=255)   tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca                                                                      >  1:532486/1‑80 (MQ=255)   tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca                                                                      >  2:532461/1‑80 (MQ=255)   tGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGcca                                                                      >  2:532421/1‑80 (MQ=255)     tCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGccaca                                                                    <  1:532422/81‑4 (MQ=255)       gACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATc                                                                 >  2:532476/1‑81 (MQ=255)        aCGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATcc                                                                >  1:532472/1‑81 (MQ=255)         cGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATccc                                                               <  1:532423/81‑1 (MQ=255)         aggAATAAAAAAAAATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATccc                                                               <  1:941309/80‑1 (MQ=255)          ggAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCg                                                              >  2:532477/1‑81 (MQ=255)                 aCCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGc                                                         >  1:532473/1‑79 (MQ=255)                  cccACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAg                                                      <  1:532424/81‑1 (MQ=255)                               cTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCAc                                         <  1:532425/81‑1 (MQ=255)                                 tCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACt                                        >  1:532479/1‑80 (MQ=255)                                  cACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACt                                        <  1:532426/79‑1 (MQ=255)                                     cAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATcc                                   <  2:532427/81‑1 (MQ=255)                                      aGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg                                  >  1:532526/1‑81 (MQ=255)                                       gTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg                                  >  1:532484/1‑80 (MQ=255)                                       gTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCg                                  >  1:532469/1‑80 (MQ=255)                                          ggTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAg                               >  2:532488/1‑80 (MQ=255)                                           gTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAgg                              <  1:532429/80‑1 (MQ=255)                                           gTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CAACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAgg                              <  1:532428/80‑1 (MQ=255)                                              ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa                          <  1:532431/81‑1 (MQ=255)                                              ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa                          <  2:532432/81‑1 (MQ=255)                                              ggTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa                          <  2:532430/81‑1 (MQ=255)                                               gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATaa                         <  2:532433/81‑1 (MQ=255)                                               gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATaa                         <  1:532434/81‑1 (MQ=255)                                               gTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATa                          >  2:532492/1‑80 (MQ=255)                                                        ccAGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGa                 >  2:532483/1‑80 (MQ=255)                                                          aGGGCAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAAt              >  1:532497/1‑81 (MQ=255)                                                              cAAGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCct          >  2:532480/1‑81 (MQ=255)                                                               aaGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctc         <  1:532435/81‑1 (MQ=255)                                                                aGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctct        <  1:532436/81‑1 (MQ=255)                                                                aGCGCCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCctct        >  1:532485/1‑81 (MQ=255)                                                                 gcgcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCtt       <  1:532437/81‑1 (MQ=255)                                                                 gcgcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCtt       <  1:532438/81‑1 (MQ=255)                                                                   gcCTAAATCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTcc     <  1:532439/81‑1 (MQ=255)                                                                        aaTCCACCG‑CCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGt  <  1:532440/79‑1 (MQ=255)                                                                                 |                                                                       GTTGTCGACGGAATAAACCCACATGTTGCGCTTCACCAGTTGGTAGGTCCAGCCGCCAGGGCAAGCGCCTAAATCCACCGCCCACATCCCGTTCGCCAGGCGTTCATCCCACTCATCCGCAGGAATAAACACATGAAATGCCTCTTCCAGT  >  NC_000913/2940521‑2940671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑