Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,989,682	(T)5→4	coding (379/483 nt)	yqeJ →	protein YqeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,989,678	0	T	.	100.0%	42.6 / NA	11	coding (375/483 nt)	yqeJ	protein YqeJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/5);  total (6/5)

TTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGATTTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATG  >  NC_000913/2989601‑2989736                                                                              |                                                           ttAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGAttt                                                          >  1:543253/1‑80 (MQ=255)    aCATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGAttttatg                                                      >  2:543254/1‑78 (MQ=255)         aaagaaagTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCtt                                                 <  1:543235/80‑1 (MQ=255)             aaagTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAg                                            <  2:543236/81‑1 (MQ=255)                      aaGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTACTCCTGA‑TTTTATGTCTTTCCAGAAAAAAgtg                                   >  2:543259/1‑81 (MQ=255)                       aGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAgtg                                   <  2:543237/80‑1 (MQ=255)                                   tGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTataata                       >  2:543263/1‑80 (MQ=255)                                        cAAGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTa                  >  2:543262/1‑80 (MQ=255)                                         aaGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTa                  >  1:543261/1‑79 (MQ=255)                                         aaGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTa                  >  2:543256/1‑79 (MQ=255)                                          aGTTGATGATGATAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAg               >  2:543247/1‑81 (MQ=255)                                               atgatgatAGACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAg           <  1:543238/80‑1 (MQ=255)                                                        gACTTGCACAACTTATTCCTGA‑TTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATg  <  2:543239/80‑1 (MQ=255)                                                                              |                                                           TTAACATCAAAGAAAGTTAATAAGTTTGAGATTATGGATCAAGTTGATGATGATAGACTTGCACAACTTATTCCTGATTTTTATGTCTTTCCAGAAAAAAGTGTAAGCTATAATATACTAAAGCAAGGTAAGCATG  >  NC_000913/2989601‑2989736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑