Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,012,483	Δ1 bp	intergenic (+90/+131)	yqeA → / ← yqeB	putative amino acid kinase YqeA/XdhC‑CoxI family protein YqeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,012,482	0	C	.	100.0%	105.7 / NA	25	intergenic (+89/+132)	yqeA/yqeB	putative amino acid kinase YqeA/XdhC‑CoxI family protein YqeB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (11/14);  total (11/14)

CCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATT  >  NC_000913/3012402‑3012558                                                                                 |                                                                             ccGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATc                                                                              <  1:547328/81‑1 (MQ=255)   gggtgggtAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATcgt                                                                            <  1:547329/81‑3 (MQ=255)    ggtgggtAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATcgtc                                                                           <  1:547330/81‑4 (MQ=255)     gtgggtAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATcgtcc                                                                          >  1:547360/1‑77 (MQ=255)      tgggtAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCt                                                                        <  2:547331/81‑1 (MQ=255)       gggtAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTa                                                                       >  1:547362/1‑81 (MQ=255)          tAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACaa                                                                    <  2:547332/81‑1 (MQ=255)            aTACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACaaa                                                                   <  2:547333/80‑1 (MQ=255)             tACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACaaaa                                                                  >  1:547378/1‑80 (MQ=255)                cATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATc                                                              <  2:547334/81‑1 (MQ=255)                        cAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTc                                                      <  1:547335/81‑1 (MQ=255)                         aaaGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCa                                                     <  1:547336/81‑1 (MQ=255)                          aaGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCaa                                                    <  1:547337/81‑1 (MQ=255)                           aGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCaa                                                    >  1:547372/1‑80 (MQ=255)                             aTTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAg                                                 <  1:547338/81‑1 (MQ=255)                               tGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGAt                                               >  1:547380/1‑81 (MQ=255)                                   aagtaagtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGc                                            >  1:547368/1‑80 (MQ=255)                                   aagtaagtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCa                                           <  2:547339/81‑1 (MQ=255)                                        agtGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGc                                       <  1:547340/80‑1 (MQ=255)                                              cTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTa                                <  2:547341/81‑1 (MQ=255)                                                ttgttgATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAgg                              >  2:547367/1‑81 (MQ=255)                                                     gATGCCGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGa                         >  2:547383/1‑81 (MQ=255)                                                         ccGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATaa                      <  2:547342/80‑1 (MQ=255)                                                         ccGGATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAg                     >  2:547376/1‑81 (MQ=255)                                                           ggATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCg                   >  1:547374/1‑81 (MQ=255)                                                           ggATGCGGCGCGAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCg                   >  1:547373/1‑81 (MQ=255)                                                                    gcgAGCGCCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATc          <  2:547343/81‑1 (MQ=255)                                                                        gcgcCTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGc      <  2:547344/81‑1 (MQ=255)                                                                            cTTAT‑CGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAtt  >  1:547370/1‑81 (MQ=255)                                                                                 |                                                                             CCGGGTGGGTAATACCATCCGGCCAAAGATTGTCAAGTAAGTGCGCTTTGTTGATGCCGGATGCGGCGCGAGCGCCTTATCCGTCCTACAAAACATCGCAAATTCAATAGATTGCAATGCACTTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATT  >  NC_000913/3012402‑3012558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑