Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,043,323	Δ1 bp	coding (301/312 nt)	yqfB ←	PUA‑like domain‑containing protein YqfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,043,321	0	T	.	100.0%	102.0 / NA	24	coding (303/312 nt)	yqfB	PUA‑like domain‑containing protein YqfB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (17/7);  total (17/7)

TTTGAGACTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGT  >  NC_000913/3043244‑3043389                                                                              |                                                                     tttGAGACTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACAttaa                                                                   <  2:554505/81‑3 (MQ=255)    gagaCTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAAtt                                                               >  2:554536/1‑81 (MQ=255)     agaCTAATCTCCTAAAAATCATGAAATTAAATGCGACATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAAtt                                                               >  2:554532/1‑80 (MQ=255)      gaCTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTc                                                              >  1:554545/1‑80 (MQ=255)       aCTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCaa                                                            >  2:554533/1‑81 (MQ=255)        cTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAAt                                                           >  2:554530/1‑81 (MQ=255)                cTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATa                                                     <  2:554506/79‑1 (MQ=255)                  aaaaaTCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAAt                                                 >  2:554528/1‑81 (MQ=255)                    aaaTCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATtg                                               >  2:554556/1‑81 (MQ=255)                    aaaTCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATtg                                               >  2:554524/1‑81 (MQ=255)                     aaTCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATtgt                                              >  1:554529/1‑81 (MQ=255)                               ttAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCgg                                    >  2:554540/1‑81 (MQ=255)                                tAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCgg                                    >  1:554531/1‑80 (MQ=255)                                 aaaTGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATGGTGTCTGACCggg                                   <  1:554507/80‑1 (MQ=255)                                      cGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGAt                             <  1:554508/81‑1 (MQ=255)                                      cGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGAt                             <  1:554509/81‑1 (MQ=255)                                        aaaTTTCAACTAACAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGt                           <  1:554510/81‑1 (MQ=255)                                                    aCAGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACtt               >  1:554534/1‑81 (MQ=255)                                                      aGGCGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACtttt             >  1:554549/1‑81 (MQ=255)                                                         cGTGAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttc          <  1:554511/81‑1 (MQ=255)                                                            gAGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcagt       >  1:554539/1‑81 (MQ=255)                                                             aGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcagtt      >  1:554550/1‑81 (MQ=255)                                                             aGTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcagtt      >  1:554537/1‑81 (MQ=255)                                                              gTTCAATTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTttcagtt      >  1:554546/1‑80 (MQ=255)                                                                  aaTTTAAAGACA‑TTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTtcagttcagt  <  1:554512/80‑1 (MQ=255)                                                                              |                                                                     TTTGAGACTAATCTCCTAAAAATCATGAAATTAAATGCGAAATTTCAACTAACAGGCGTGAGTTCAATTTAAAGACATTTAAATTCAATCACATAAAATTGTGTCTGACCGGGATAGATGTCAGCAATGACTTTTTTCAGTTCAGT  >  NC_000913/3043244‑3043389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑