Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,250	Δ1 bp	coding (801/930 nt)	yhcC ←	radical SAM family oxidoreductase YhcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,248	0	G	.	100.0%	149.8 / NA	35	coding (803/930 nt)	yhcC	radical SAM family oxidoreductase YhcC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (23/12);  total (23/12)

GCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAG  >  NC_000913/3353169‑3353324                                                                                |                                                                             gCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCgg                                                                             >  2:626897/1‑81 (MQ=255)     tGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCggcgac                                                                         <  2:626885/81‑5 (MQ=255)         cattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGgc                                                                    >  2:626937/1‑81 (MQ=255)         cattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGgc                                                                    >  1:626939/1‑81 (MQ=255)                  aGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCgg                                                            >  2:626966/1‑80 (MQ=255)                  aGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCgg                                                            >  1:626945/1‑80 (MQ=255)                    cGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGaaa                                                         >  2:626958/1‑81 (MQ=255)                     gATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAAt                                                        >  2:626929/1‑81 (MQ=255)                     gATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAAt                                                        >  1:626956/1‑81 (MQ=255)                          aGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCggt                                                   >  1:626936/1‑81 (MQ=255)                          aGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCggt                                                   >  1:626944/1‑81 (MQ=255)                           gCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCggtg                                                  <  1:626887/81‑1 (MQ=255)                           gCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCggtg                                                  >  1:626950/1‑81 (MQ=255)                           gCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCggtg                                                  <  1:626886/81‑1 (MQ=255)                               gACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTa                                               <  2:626888/80‑1 (MQ=255)                                 ccATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGAt                                            <  1:626889/81‑1 (MQ=255)                                   aTTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCa                                          >  1:626955/1‑81 (MQ=255)                                     tCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACt                                        >  2:626938/1‑81 (MQ=255)                                      cccGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACt                                        <  1:626890/80‑1 (MQ=255)                                       ccGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTc                                      >  1:626951/1‑81 (MQ=255)                                        cGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTcc                                     <  1:626891/81‑1 (MQ=255)                                                gATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCg                              >  1:626983/1‑80 (MQ=255)                                                gATTTTCGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCg                              <  1:626892/80‑1 (MQ=255)                                                  ttttCTCGCCACAGCGGAGCAAGCAGCGTC‑GGCGACAGGCGCTGGCGGAAATGCGGTGGTAAATCACTTCCGGTGGCGTAt                           <  2:626893/81‑1 (MQ=255)                                                      cGCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGc                        >  2:626949/1‑80 (MQ=255)                                                       gCACCACAGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCg                       >  1:627023/1‑80 (MQ=255)                                                            acaGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAt                 <  1:626894/81‑1 (MQ=255)                                                              aGCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCAttt               <  2:626895/81‑1 (MQ=255)                                                               gCGGAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTtc              <  1:626896/81‑1 (MQ=255)                                                                 ggAGCAAGCAGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTtctc            >  1:626948/1‑81 (MQ=255)                                                                   agcaagcaGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCg          >  1:626946/1‑81 (MQ=255)                                                                    gcaagcaGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCgg         >  2:626941/1‑81 (MQ=255)                                                                    gcaagcaGCGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCgg         >  2:626942/1‑81 (MQ=255)                                                                        gcagcGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt     <  1:626897/81‑1 (MQ=255)                                                                        gcagcGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt     >  1:626947/1‑81 (MQ=255)                                                                         cagcGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGt     >  2:626943/1‑80 (MQ=255)                                                                           gcGTC‑GGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAg  >  1:626954/1‑81 (MQ=255)                                                                                |                                                                             GCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAG  >  NC_000913/3353169‑3353324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑