Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,970	Δ1 bp	coding (45/717 nt)	yhcF →	DUF1120 domain‑containing protein YhcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,969	0	C	.	100.0%	101.2 / NA	24	coding (44/717 nt)	yhcF	DUF1120 domain‑containing protein YhcF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (13/11);  total (13/11)

TTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCC  >  NC_000913/3366892‑3367041                                                                              |                                                                         ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGcatg                                                                       <  1:630095/81‑4 (MQ=255)       gCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAgcg                                                                <  2:630096/81‑1 (MQ=255)        cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAgcg                                                                >  1:630136/1‑80 (MQ=255)         aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAgcgc                                                               >  2:630127/1‑80 (MQ=255)                 ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCt                                                       >  1:630138/1‑80 (MQ=255)                 ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTg                                                      <  2:630097/81‑1 (MQ=255)                     cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATa                                                   <  2:630098/80‑1 (MQ=255)                       ggtggAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATagag                                                >  2:630131/1‑81 (MQ=255)                              gCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                         >  2:630132/1‑81 (MQ=255)                               cAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAAtt                                        >  2:630134/1‑81 (MQ=255)                                aaaaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                       <  1:630099/81‑1 (MQ=255)                                aaaaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                       <  2:630101/81‑1 (MQ=255)                                aaaaTGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTg                                       <  1:630100/81‑1 (MQ=255)                                          aTGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                             >  1:630142/1‑81 (MQ=255)                                          aTGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGt                             <  1:630102/81‑1 (MQ=255)                                            gTAAAATTACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGa                           >  1:630147/1‑81 (MQ=255)                                                  ttACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTgg                     <  2:630103/81‑1 (MQ=255)                                                  ttACTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTg                      >  1:630140/1‑80 (MQ=255)                                                    aCTGATTGCCGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTgg                     >  1:630143/1‑79 (MQ=255)                                                            ccGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGc           >  1:630141/1‑81 (MQ=255)                                                             cGGAAGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCt          <  1:630104/81‑1 (MQ=255)                                                                aaGTGCCTTTTTTG‑GATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg        >  2:630137/1‑80 (MQ=255)                                                                 aGTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa      >  1:630146/1‑81 (MQ=255)                                                                  gTGCCTTTTTTG‑CATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTAc     <  2:630105/81‑1 (MQ=255)                                                                     ccTTTTTTG‑CATGTCAACGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTcc  <  1:630106/81‑1 (MQ=255)                                                                              |                                                                         TTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCC  >  NC_000913/3366892‑3367041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑