Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,588,752	Δ1 bp	coding (429/1071 nt)	ugpC ←	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,588,751	0	C	.	100.0%	285.4 / NA	65	coding (430/1071 nt)	ugpC	sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (27/38);  total (27/38)

AGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCC  >  NC_000913/3588675‑3588827                                                                             |                                                                             aGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGcacac                                                                          >  2:683475/1‑77 (MQ=255)     tGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTg                                                                     >  2:683490/1‑81 (MQ=255)     tGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTg                                                                     >  1:683494/1‑81 (MQ=255)      ggCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCt                                                                      <  2:683399/79‑1 (MQ=255)      ggCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTgg                                                                    >  2:683489/1‑81 (MQ=255)       gCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTgg                                                                    <  2:683401/80‑1 (MQ=255)       gCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGgc                                                                   <  1:683402/81‑1 (MQ=255)       gCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGgc                                                                   <  1:683400/81‑1 (MQ=255)            gAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTg                                                               <  1:683403/80‑1 (MQ=255)            gAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGa                                                              >  2:683468/1‑81 (MQ=255)             aGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAc                                                             >  2:683497/1‑81 (MQ=255)             aGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAc                                                             <  2:683404/81‑1 (MQ=255)              ggTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAcc                                                            <  2:683406/81‑1 (MQ=255)              ggTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAcc                                                            >  2:683481/1‑81 (MQ=255)              ggTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAcc                                                            <  2:683407/81‑1 (MQ=255)              ggTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAcc                                                            <  2:683405/81‑1 (MQ=255)                ttAGAGAGCGGCTCATCAAACAGGAACACCGCC‑GATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgcc                                                         <  2:683408/81‑1 (MQ=255)                 tAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgcc                                                         >  2:683474/1‑81 (MQ=255)                  agagagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgccg                                                        >  2:683482/1‑81 (MQ=255)                  agagagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgccg                                                        >  2:683485/1‑81 (MQ=255)                  agagagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgccg                                                        >  1:683461/1‑81 (MQ=255)                   gagagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGAccgccg                                                        <  1:683409/80‑1 (MQ=255)                    agagCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGa                                                      <  2:683410/81‑1 (MQ=255)                        cGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGc                                                  <  1:683411/81‑1 (MQ=255)                         ggCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCt                                                 <  1:683413/81‑1 (MQ=255)                         ggCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCt                                                 <  2:683412/81‑1 (MQ=255)                          gCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTc                                                <  2:683414/81‑1 (MQ=255)                           cTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTcg                                               >  2:683472/1‑81 (MQ=255)                                   aCAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGc                                        >  1:683510/1‑80 (MQ=255)                                    cAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGa                                      <  2:683415/81‑1 (MQ=255)                                    cAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGa                                      <  1:683416/81‑1 (MQ=255)                                     aGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGAc                                     <  1:683417/81‑1 (MQ=255)                                       gAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGt                                   >  2:683499/1‑81 (MQ=255)                                        aaCACCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGtt                                  >  1:683479/1‑81 (MQ=255)                                         acacCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGttt                                 <  2:683419/81‑1 (MQ=255)                                         acacCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGttt                                 <  1:683418/81‑1 (MQ=255)                                          cacCGCCGGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTg                                <  2:683420/81‑1 (MQ=255)                                            ccgccgGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAg                              <  2:683421/81‑1 (MQ=255)                                             cgccgGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAg                              >  1:683562/1‑80 (MQ=255)                                              gccgGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAg                              >  1:683486/1‑79 (MQ=255)                                                cgGATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGa                          >  1:683478/1‑81 (MQ=255)                                                  gATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGAcc                        <  2:683422/81‑1 (MQ=255)                                                  gATCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGAcc                        <  1:683423/81‑1 (MQ=255)                                                   aTCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCg                       >  2:683502/1‑81 (MQ=255)                                                    tCGCGCACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGt                      <  2:683424/81‑1 (MQ=255)                                                      gcgcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTc                     <  2:683426/80‑1 (MQ=255)                                                      gcgcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTc                     >  2:683498/1‑80 (MQ=255)                                                      gcgcACAATCGCGCGGCCCATCG‑CACACGCTAGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTcc                    <  2:683425/81‑1 (MQ=255)                                                       cgcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCa                   <  1:683428/81‑1 (MQ=255)                                                       cgcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCa                   <  2:683427/81‑1 (MQ=255)                                                        gcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAg                  >  1:683496/1‑81 (MQ=255)                                                        gcACAATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAg                  >  1:683484/1‑81 (MQ=255)                                                          acaATCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCt                <  1:683429/81‑1 (MQ=255)                                                            aaTCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTcc              <  1:683430/81‑1 (MQ=255)                                                             aTCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTc               >  1:683487/1‑79 (MQ=255)                                                             aTCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCa             <  2:683432/81‑1 (MQ=255)                                                             aTCGCGCGGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCa             <  2:683431/81‑1 (MQ=255)                                                                gcgcgGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGaa          >  2:683515/1‑81 (MQ=255)                                                                 cgcgGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAAt         >  2:683488/1‑81 (MQ=255)                                                                   cgGCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgc       >  1:683491/1‑81 (MQ=255)                                                                    ggCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcg      >  1:683493/1‑81 (MQ=255)                                                                    ggCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcg      >  1:683504/1‑81 (MQ=255)                                                                    ggCCCATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATgcg      <  1:683433/81‑1 (MQ=255)                                                                        cATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgcc  <  1:683435/81‑1 (MQ=255)                                                                        cATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgcc  <  1:683434/81‑1 (MQ=255)                                                                        cATCG‑CACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGcgcc  <  2:683436/81‑1 (MQ=255)                                                                             |                                                                             AGCTTGGCATCGAGGTTAGAGAGCGGCTCATCAAACAGGAACACCGCCGGATCGCGCACAATCGCGCGGCCCATCGCCACACGCTGGCGCTGACCGCCGGAAAGCTCGCGCGGGCGACGTTTGAGCAGACCGTCCAGCTCCAGAATGCGCGCC  >  NC_000913/3588675‑3588827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑